MRI: Track 1 Acquisition of a 600MHz NMR Spectrometer for Research, Teaching, and Outreach

该奖项由主要研究仪器和化学研究仪器计划以及刺激竞争性研究的既定计划共同支持。蒙大拿大学正在购买一台600 MHz核磁共振（NMR）光谱仪，该光谱仪配备了三重共振探头、双共振宽带探头、变温装置和自动进样器，以支持布鲁斯·鲍勒教授及其同事（Klara Briknarova、Orion Berryman、Travis Hughes和丹尼尔·迪卡托）的研究。该仪器促进了结构生物学、生物物理学、超分子化学、有机化学、无机化学和药物化学领域的研究。一般来说，NMR光谱是化学家用来阐明分子结构的最有力的工具之一。它用于识别未知物质，表征分子内原子的特定排列，并研究溶液或固态分子之间相互作用的动力学。对于从事前沿研究的化学家来说，使用最先进的NMR光谱仪是必不可少的。该仪器增强了蒙大拿大学几个部门各级学生的教育，研究和教学工作。它将直接影响12个实验室的研究，并将影响学生在两个上师实验室课程和几个研究生课程。新的仪器也将提供给蒙大拿州和周边州的地区部落学院使用。该奖项旨在支持各级研究和教育。耦合到三重共振探针的600 MHz NMR光谱仪将用于评估稳定蛋白质的合理突变，并用于研究纤连蛋白的结构和动力学。双共振宽带探针将用于研究工作，采用氟核磁共振研究核受体。该探针还将通过质子-氟NMR相关实验加强对非生物折叠体的研究。变温能力将扩大围绕瞬时高价第一行过渡金属络合物的研究范围，旨在激活碳-氢键。自动进样器将允许使用饱和转移差（STD）NMR对配体蛋白相互作用进行高通量评价，阐明与C型凝集素受体的基本配体结合事件。600 MHz NMR系统还将为药物化学、天然产物、材料科学和聚合物化学等学科的各种用户提供服务。为了应对全球氦气供应的挑战，该仪器的采购包括一个超导磁体，其低损耗低温恒温器可减少该机构的氦气消耗。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs, and the Established Program to Stimulate Competitive Research. The University of Montana is acquiring a 600 MHz Nuclear Magnetic Resonance (NMR) spectrometer equipped with a triple-resonance probe, a double-resonance broadband probe, a variable temperature unit, and an automated sample changer to support the research of Professor Bruce Bowler and his colleagues: Klara Briknarova, Orion Berryman, Travis Hughes, and Daniel Decato. This instrument facilitates research in the areas of structural biology, biophysics, supramolecular chemistry, organic chemistry, inorganic chemistry, and medicinal chemistry. In general, NMR spectroscopy is one of the most powerful tools available to chemists for the elucidation of the structure of molecules. It is used to identify unknown substances, to characterize specific arrangements of atoms within molecules, and to study the dynamics of interactions between molecules in solution or in the solid state. Access to state-of-the-art NMR spectrometers is essential to chemists who are carrying out frontier research. This instrument enhances the educational, research, and teaching efforts of students at all levels in several departments at the University of Montana. It will directly impact the research of 12 laboratories and will impact students in two upper division lab courses and several graduate courses. The new instrument will also be available for use by regional tribal colleges in Montana and surrounding states.This award is aimed at supporting research and education at all levels. The 600 MHz NMR spectrometer coupled to the triple-resonance probe will be used for evaluating rational mutations for stabilizing proteins and for studying the structure and dynamics of Fibronectin. The double resonance broadband probe will serve research efforts employing fluorine NMR to study nuclear receptors. This probe will also enhance studies of abiotic foldamers through proton-fluorine NMR correlation experiments. The variable temperature capabilities will expand the scope of the studies surrounding transient high valent first row transition metal complexes designed to activate carbon-hydrogen bonds. The autosampler will permit high throughput evaluations of ligand protein interactions using saturation-transfer difference (STD) NMR elucidating fundamental ligand binding events with C-type Lectin receptors. The 600 MHz NMR system will also serve a variety of users within the disciplines of medicinal chemistry, natural products, materials science, and polymer chemistry. In response to global helium supply challenges, the acquisition of this instrument includes a superconducting magnet with a low loss cryostat reducing the institution’s consumption of helium.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.