Studies of protein-heparin binding via Frontal Analysis Continuous Capillary Electrophoresis (FACCE), FACCE/Electrospray-MS, and protein electrostatic modeling

通过前沿分析连续毛细管电泳 (FACCE)、FACCE/Electrospray-MS 和蛋白质静电建模研究蛋白质-肝素结合

• 批准号: 0619039
• 负责人: Paul Dubin
• 金额: --
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0619039&HistoricalAwards=false
• 关键词: Studies; protein; heparin; binding; via

Professor Paul Dubin of Indiana University-Purdue University at Indianapolis is supported by the Analytical and Surface Chemistry Program to study the molecular basis of glycosaminoglycans (GAGs)-protein interactions. GAGs are strongly anionic polysaccharides that constitute the major components of proteoglycans, and they are important in the diverse functions of this class of proteins. Some of the important functions are wound repair and tumor formation. The approach is to use Frontal analysis continuous caplliary electrophoresis (FACCE). The major challenge is the heterogeneity of GAGs, thus the study pushes the limits of separation and characterization techniques. The work provides interdisciplinary training to students in the areas of analytical chemistry, cell biology, biophysical chemistry and computational chemistry. The PI collaborates with J. Dage from Eli Lilly Research Central Research for the use of MS-TOF instruments.A better understanding of structure/function relationships of gylcosaminoglycan-protein interactions will improve the design and efficacy of heparin-based drugs, which belong to one of the most prescribed drug categories (anticoagulants and antithrombotics).

印第安纳大学-普渡大学的Paul Dubin教授受到分析和表面化学项目的支持，研究糖胺聚糖(GAGs)-蛋白质相互作用的分子基础。GAGs是一种强阴离子多糖，是蛋白质多糖的主要成分，在这类蛋白质的多种功能中起着重要作用。一些重要的功能是伤口修复和肿瘤形成。方法是使用正面分析连续毛细管电泳（face）。主要的挑战是gag的异质性，因此该研究推动了分离和表征技术的极限。这项工作为学生提供分析化学、细胞生物学、生物物理化学和计算化学领域的跨学科培训。PI与来自礼来研究中心的J. Dage合作使用MS-TOF仪器。更好地理解葡萄糖胺聚糖-蛋白相互作用的结构/功能关系将改善肝素类药物的设计和疗效，肝素类药物属于处方最多的药物类别之一（抗凝血剂和抗血栓药）。