Aqueous Size-Exclusion Chromatography

大分子经常通过尺寸排阻分析 层析 小分子保留在 固定介质，而较大的分子直接洗脱。 杜宾教授，在一个由分析和 化学系表面化学专业， 研究影响分离的其他因素， 大小排阻原理。 一旦一个分子进入一个孔，它与表面的相互作用， 这些孔以一种足以使当前理论复杂方式 无法解释分离过程的本质。 工作 将在该补助金下进行的调查将调查各种 物理和化学相互作用机制 大分子和普通多孔介质的表面， 为尺寸排阻设计提供了合理依据 大分子混合物的分离。

Macromolecules are frequently analyzed by size-exclusion chromatography. Small molecules are retained in the pores of a stationary medium, while larger molecules directly are eluted. Prof. Dubin, in a program supported by the Analytical and Surface Chemistry Program of the Chemistry Division, is studying additional factors which effect separations based on the size-exclusion principle. Once a molecule enters a pore, it interacts with the surface of the pore in a manner sufficiently complex that current theory cannot explain the nature of the separation process. The work to be carried out under this grant will investigate a variety of physical and chemical interaction mechanisms between macromolecules and the surface of common porous media, thus providing a rational basis for the design of size-exclusion separations of macromolecular mixtures.