Antimicrobial Peptide Defenses in Amphibian Skin

生物学家对新的“沉默的春天”的可能性感到震惊。 这一次，鸟儿会唱歌，但许多常见的青蛙和蟾蜍（两栖动物）可能不会在晚上唱歌。 杀死青蛙和蟾蜍的潜在杀手是一种侵入皮肤的真菌。 一些两栖类物种似乎对真菌更具抵抗力，因为它们能够分泌小的保护蛋白（抗菌肽）到皮肤表面。 这项研究将确定健康两栖动物产生的抗菌肽的量，肽释放到皮肤上后的恢复速度，以及肽在皮肤上保持有效的时间。 一些实验将测试洗掉或耗尽肽是否会使两栖动物更容易受到真菌的影响。 这种真菌本身可以引起青蛙产生更多的抗菌肽，或者皮肤损伤可能导致产生更少的保护性肽。 这项研究将测量肽量在暴露于真菌或细菌后是否增加或减少。 青蛙和蟾蜍可以强调由于拥挤或其他环境变化，包括寒冷的温度。 这项研究将确定这些自然压力如何影响抗菌肽的产生和对真菌的敏感性。 研究结果可能会建议更好的战略，野生动物物种的管理，以保护他们。 它将培养学生在免疫学和保护领域。

Biologists are alarmed by the possibility of a new "silent spring". This time the birds will be singing, but many of the common frogs and toads (amphibians) may not be singing at night. The killer that has the potential to silence the frogs and toads is a fungus that invades the skin. Some amphibian species seem to be more resistant to the fungus due to their ability to secrete small protective proteins (antimicrobial peptides) onto the skin surface. This study will determine the amount of antimicrobial peptides made by healthy amphibians, the rate of restoration after the peptides are discharged onto the skin, and the time the peptides remain effective on the skin. Some experiments will test whether washing away or depleting the peptides will make the amphibians more susceptible to the fungus. The fungus, itself, could provoke a response from the frog to make more antimicrobial peptides, or the injury to the skin could result in production of less of the protective peptides. This study will measure whether peptide amounts increase or decrease after exposure to the fungus or to bacteria. Frogs and toads can be stressed due to crowding or other environmental changes including cold temperature. This study will determine how these natural stresses affect production of antimicrobial peptides and susceptibility to the fungus. Results may suggest better strategies for management of wildlife species to preserve them. It will train students in the field of immunology and conservation.