Collaborative Research: ATOL: A Phylogenetic and Genomic Investigation of the Algal Heterokont Tree

合作研究：ATOL：藻类异质树的系统发育和基因组研究

• 批准号: 0629554
• 负责人: Matthew Julius
• 金额: $ 25.53万
• 依托单位: St. Cloud State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0629554&HistoricalAwards=false
• 关键词: Collaborative; Research; ATOL; Phylogenetic; Genomic

An award has been made to Robert A. Andersen (Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine), Rose Ann Cattolico and Gabrielle Rocap (University of Washington, Seattle, Washington), Robert Jansen, Elizabeth Ruck and Edward Theriot (University of Texas, Austin Texas), Matt Julius (St. Cloud State University, St. Cloud, Minnesota), Stefano Draisma (National Herbarium and Leiden University, Leiden, The Netherlands) and Hiroshi Kawai (Kobe University, Kobe, Japan) to determine the relationships among the heterokont classes by generating two large molecular data sets. Currently, 14 taxonomic classes of heterokont algae are recognized, and they include over 100,000 described species (as many as one million total species has been estimated by experts). Common members include brown seaweeds (e.g., kelp) and the diatoms. Remarkably, despite two centuries of light microscopic study, 50 years of electron microscopic study, and 20 years of molecular investigations, the phylogenetic relationships among the 14 classes remains unknown. DNA sequences of seven nuclear, mitochondrial, and chloroplast genes from 270 heterokont algal species and 30 nonalgal relatives will be obtained and, entire chloroplast genomes from 30 species will be sequenced. The genome sequences will provide important new data for understanding chloroplasts and their origin(s), especially since knowledge in this field is greatly biased toward land plants. Algae serve as the base of the food chain for all organisms that dwell in the ocean and account for approximately half of the global photosynthesis, and therefore they provide approximately 50% of the oxygen we breathe. Members of the heterokont algae vary in morphology from simple unicells to highly complex seaweeds. Brown algae are the principal elements of seaweed beds and these macroalgae have high economic values as food and biomass resources. A number of heterokont algae are harmful to marine life. For example, some algae produce toxins that when concentrated in shellfish kill humans, while other species of heterokonts are well-known fish killers. Conversely, other heterokonts serve as a human food source, they are used in industrial processes and they have found new recognition in the nanotechnology industry. The closest relatives to the heterokont algae are the pseudofungi, which include many plant pathogens, such as the fungus that caused the Irish potato famine, and various molds and mildews. Graduate and undergraduate students and postdoctoral fellows from diverse cultural and ethnic backgrounds will be supported and trained in a wide diversity of fields, including evolution, genomics, molecular biology, computational biology, and plant biology. the research will be integrated with K-12 educators, public museums and parks using already established programs at several of the participating institutions. A Deep Brown web site already has been established, and it will be greatly expanded as part of this project.

获奖者包括 Robert A. Andersen（毕格罗海洋科学实验室，缅因州西布斯贝港）、Rose Ann Cattolico 和 Gabrielle Rocap（华盛顿大学，西雅图，华盛顿）、Robert Jansen、Elizabeth Ruck 和 Edward Theriot（德克萨斯大学，德克萨斯州奥斯汀）、Matt Julius（圣克劳德州立大学，明尼苏达州圣克劳德）、Stefano Draisma（国家植物标本馆和莱顿大学，荷兰莱顿）和 Hiroshi Kawai（神户大学，日本神户）通过生成两个大型分子数据集来确定异质体类别之间的关系。 目前，已识别出 14 个异质藻类分类学类别，其中包括超过 100,000 个已描述的物种（专家估计总数多达 100 万个）。 常见成员包括棕色海藻（例如海带）和硅藻。 值得注意的是，尽管经过两个世纪的光学显微镜研究、50 年的电子显微镜研究和 20 年的分子研究，这 14 个纲之间的系统发育关系仍然未知。将获得来自 270 种异质藻类和 30 种非藻类近缘种的 7 个核基因、线粒体基因和叶绿体基因的 DNA 序列，并对 30 个物种的整个叶绿体基因组进行测序。基因组序列将为了解叶绿体及其起源提供重要的新数据，特别是因为该领域的知识极大地偏向于陆地植物。藻类是海洋中所有生物体食物链的基础，约占全球光合作用的一半，因此它们提供了我们呼吸的约 50% 的氧气。 异质藻类成员的形态各异，从简单的单细胞到高度复杂的海藻。褐藻是海藻床的主要成分，这些大型藻类作为食物和生物质资源具有很高的经济价值。 许多异质藻类对海洋生物有害。例如，一些藻类产生的毒素在贝类中浓缩时会杀死人类，而其他种类的异质藻则是众所周知的鱼类杀手。相反，其他异质体可作为人类食物来源，用于工业过程，并在纳米技术行业中得到了新的认可。与异质藻类最接近的亲戚是假真菌，其中包括许多植物病原体，例如引起爱尔兰马铃薯饥荒的真菌以及各种霉菌和霉菌。来自不同文化和种族背景的研究生和本科生以及博士后研究员将在进化、基因组学、分子生物学、计算生物学和植物生物学等广泛领域得到支持和培训。该研究将利用几个参与机构已经制定的计划，与 K-12 教育工作者、公共博物馆和公园结合起来。 Deep Brown 网站已经建立，作为该项目的一部分，它将得到极大的扩展。