Collaborative Research: AToL: ACCESS DNA viruses: A Comprehensive survey of Circular Eukaryotic Single-Stranded DNA viruses in Invertebrates and Fungi

合作研究：AToL：ACCESS DNA 病毒：无脊椎动物和真菌中环状真核单链 DNA 病毒的综合调查

• 批准号: 1239976
• 负责人: Mya Breitbart
• 金额: $ 99.54万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-01 至 2018-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1239976&HistoricalAwards=false
• 关键词: Collaborative; Research; AToL; ACCESS; DNA

Viruses affect all forms of cellular life; however, it is extremely difficult to trace their evolutionary history. This project will reconstruct the phylogeny of an understudied branch of the viral tree of life: that of circular eukaryotic single-stranded DNA (CESS) viruses. CESS viruses are the smallest known viruses, yet they include pathogens with devastating impacts on agriculture worldwide. Recently, general environmental sampling has uncovered a diversity of CESS viruses that are only distantly related to these pathogens and has shown that they infect a wider range of hosts than previously thought, including insects and fungi. This project systematically tests previously unexplored hosts to discover, sequence, and classify their CESS viruses. Phylogenetic analyses will then determine how all CESS viruses are related to one another, providing a framework for classifying the CESS viral tree of life.Broader impacts of this research include training of graduate students, undergraduates, and postdocs, as well as K-12 outreach and creation of web-based resources for the scientific and general public. Middle school students will be involved in collecting invertebrate samples, which will be processed during a virology training workshop for graduate students and postdocs on CESS virus genomics. Project results will be displayed on a website to increase public knowledge about CESS viruses, and bioinformatics tools will be shared freely with the scientific community.

病毒影响所有形式的细胞寿命；但是，很难追踪他们的进化史。该项目将重建生命病毒树的研究研究的系统发育：圆形真核生物单链DNA（CESS）病毒的系统发育。 CESS病毒是最小的已知病毒，但它们包括对全球农业有破坏性影响的病原体。最近，一般的环境抽样发现了多种与这些病原体相关的CESS病毒多样性，并表明它们感染了比以前想象的更广泛的宿主，包括昆虫和真菌。该项目系统地测试了先前未开发的宿主，以发现，顺序和对其CESS病毒进行分类。然后，系统发育分析将确定所有CESS病毒之间的关系如何相互关联，提供了一个框架，以对CESS病毒树的生命树进行分类。这项研究的影响包括培训研究生，本科生和博士学位，以及K-112的培训，以及K-112的培训，以及K-112的介绍和为科学和公众提供网络基础资源。中学生将参与收集无脊椎动物样本，该样本将在研究生的病毒学培训讲习班和CESS病毒基因组学博士后进行处理。项目结果将在网站上显示，以增加公众对CESS病毒的了解，并且生物信息学工具将与科学界自由共享。