Threading Interdisciplinary Biological-Chemistry Experiments Throughout the Undergraduate Chemistry Curriculum using MALDI Mass Spectrometry

使用 MALDI 质谱将跨学科生物化学实验贯穿整个本科化学课程

• 批准号: 0633286
• 负责人: Douglas Beussman
• 金额: --
• 依托单位: Saint Olaf College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0633286&HistoricalAwards=false
• 关键词: Threading; Interdisciplinary; Biological; Chemistry; Experiments

Chemistry (12). This project incorporates matrix-assisted laser desorption/ionization (MALDI) mass spectrometry throughout the chemistry curriculum in order to study the large bio-molecules that are part of an interdisciplinary chemistry-biology interface. The MALDI instrument is a key component in a new Bioanalytical Chemistry course that prepares students for research at this chemistry-biology interface. MALDI, an important analytical tool in a wide range of research areas, is incorporated into a Forensic Science course, a new introductory chemistry-biology integrated course, and several other courses. This incorporation includes adapting labs developed by other institutions using MALDI instrumentation as well as developing new labs for Bioanalytical Chemistry, Instrumental Analysis, and Forensic Science. The incorporation of MALDI throughout the curriculum insures maximum use of the instrument while preparing students for future research in chemical or biological fields. Students' experiences using MALDI, their understanding of the MALDI technique with its abilities and limitations, and the impact on student learning is measured using on-line evaluations. Senior peer evaluation is used to evaluate the new MALDI experiments. The project demonstrates how to use the MALDI-TOF in all levels of the chemistry curriculum.

化学(12)。该项目将基质辅助激光解吸/电离（MALDI）质谱法纳入整个化学课程，以研究作为跨学科化学-生物学界面一部分的大型生物分子。MALDI仪器是新生物分析化学课程的关键组成部分，为学生在这一化学-生物学界面的研究做好准备。MALDI是广泛研究领域的重要分析工具，被纳入法医学课程、新的化学-生物学综合导论课程和其他几门课程。这种整合包括调整其他机构使用MALDI仪器开发的实验室，以及开发生物分析化学、仪器分析和法医学的新实验室。将MALDI纳入整个课程，确保最大限度地利用该仪器，同时为学生将来在化学或生物领域的研究做好准备。学生使用MALDI的经验，他们对MALDI技术及其能力和局限性的理解，以及对学生学习的影响是通过在线评估来衡量的。采用高级同伴评价对新的MALDI实验进行评价。该项目演示了如何在化学课程的各个阶段使用MALDI-TOF。