SBIR Phase I: Spiral Countercurrent Chromatography for Biotechnology

SBIR 第一阶段：用于生物技术的螺旋逆流色谱

• 批准号: 0638082
• 负责人: MARTHA KNIGHT
• 金额: $ 10万
• 依托单位: CC Biotech LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0638082&HistoricalAwards=false
• 关键词: SBIR; Phase; Spiral; Countercurrent; Chromatography

This Small Business Innovation Research (SBIR) Phase I project focuses on a new design of a spiral flow-through separation coil for planetary centrifuge chromatographs that are already commercialized for natural products and synthetic small molecule purification. This scientific and engineering project will extend the advantageous high resolution, solid support-free, highly scaleable process to the biotechnology field. Success in purification of high molecular weight compounds by countercurrent chromatography will lower the manufacturing costs of biotechnology products, including therapeutics. Improvement in the separation process for the purification of more complex therapeutic products is needed in the industry. An all-liquid process that can purify in high yield, compounds in any solvent system designed for their solubility is extended to the larger MW compounds, including biological molecules. The proposed design retains the more viscous and heavy two-phase solvent systems that are suitable for proteins, peptides and particles, and prevent denaturation - thereby enabling the recovery of high activity.

这个小型企业创新研究（SBIR）第一阶段项目的重点是一个新的设计的螺旋流通分离线圈的行星式离心色谱仪，已经商业化的天然产品和合成小分子纯化。该科学和工程项目将把具有优势的高分辨率、无固体载体、高度可扩展的过程扩展到生物技术领域。通过逆流色谱法纯化高分子量化合物的成功将降低生物技术产品（包括治疗剂）的制造成本。在工业中需要改进用于纯化更复杂的治疗产品的分离方法。一个全液体的过程，可以在高产量纯化，化合物在任何溶剂系统设计为他们的溶解性扩展到较大的分子量的化合物，包括生物分子。所提出的设计保留了更粘稠和更重的两相溶剂系统，适用于蛋白质，肽和颗粒，并防止变性-从而能够恢复高活性。