SBIR Phase I: Spiral Countercurrent Chromatography for Biotechnology

这个小型企业创新研究（SBIR）第一阶段项目的重点是一种用于行星离心色谱仪的螺旋流分离线圈的新设计，该色谱仪已经用于天然产品和合成小分子纯化的商业化。这一科学和工程项目将把高分辨率、无固支撑、高度可扩展的工艺扩展到生物技术领域。逆流色谱法纯化高分子量化合物的成功将降低生物技术产品（包括治疗药物）的制造成本。工业中需要改进分离工艺以纯化更复杂的治疗产品。一种可以在任何溶剂系统中以高收率纯化化合物的全液体工艺扩展到更大分子量的化合物，包括生物分子。提出的设计保留了更粘稠和更重的两相溶剂系统，适用于蛋白质，肽和颗粒，并防止变性-从而能够恢复高活性。

This Small Business Innovation Research (SBIR) Phase I project focuses on a new design of a spiral flow-through separation coil for planetary centrifuge chromatographs that are already commercialized for natural products and synthetic small molecule purification. This scientific and engineering project will extend the advantageous high resolution, solid support-free, highly scaleable process to the biotechnology field. Success in purification of high molecular weight compounds by countercurrent chromatography will lower the manufacturing costs of biotechnology products, including therapeutics. Improvement in the separation process for the purification of more complex therapeutic products is needed in the industry. An all-liquid process that can purify in high yield, compounds in any solvent system designed for their solubility is extended to the larger MW compounds, including biological molecules. The proposed design retains the more viscous and heavy two-phase solvent systems that are suitable for proteins, peptides and particles, and prevent denaturation - thereby enabling the recovery of high activity.