SBIR Phase I: Digital Barcode Etched Assayable Micro (BEAM) Bead for Rare Biomolecules Separation and Purification

这个小企业创新研究（SBIR）第一阶段项目的重点是开发一种检测突变的方法，该方法结合了条形码珠，微流体和核酸杂交技术，用于分离和纯化体液中的多种生物标志物。血液中生物标志物或突变循环DNA的存在可以是诊断和预后指标，使体液可能用于诊断和患者监测。所提出的技术允许从多重扩增测定中选择性富集超罕见突变等位基因。 由于已经证明在癌症患者的血液中存在非常少量的生物标志物或肿瘤DNA循环，因此该技术可以开发自动血液检测，用于早期检测高危患者的微小残留疾病。

This Small Business Innovation Research (SBIR) Phase I project focuses on developing a process to detect mutations that combines the technologies of bar-coded beads, microfluidic, and nucleic acid hybridization for the separation and purification of multiple biomarkers in bodily fluids. The presence of biomarkers or mutant circulating DNA in blood can be a diagnostic and prognostic indicator, making body fluids potentially useful for diagnostics and patient monitoring. The proposed technology allows for the selective enrichment of ultra rare mutant alleles from a multiplexed amplification assay. As it has been shown that there are biomarkers, or tumor DNA circulating in a very small quantity in the blood of cancer patients, this technology may allow the development of automated blood tests for early detection of minimal residual diseases in high-risk patients.