RUI: Examination of the Genetic Mechanisms Used to Control the Expression of Salinity-regulated Proteins in the Archaeon Haloferax volcanii

RUI：用于控制古细菌 Haloferax volcanii 中盐度调节蛋白表达的遗传机制的检查

• 批准号: 0641243
• 负责人: Kelly Bidle
• 金额: --
• 依托单位: Rider University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0641243&HistoricalAwards=false
• 关键词: RUI; Examination; Genetic; Mechanisms; Used

This project aims to employ a broad variety of proteomic, genetic, and bioinformatic techniques for use in studies that will further characterize those genes, and their corresponding functions, necessary for adaptation to variable salinities in the model haloarchaeon, Haloferax volcanii. Proteomic analyses have led to the discovery of a set of salinity-regulated proteins in Hfx. volcanii that now await detailed characterization of the mechanisms involved in their regulation. Among the proteins identified were a bacterial-like transcriptional activator that is regulated by NaCl concentrations at the level of transcription. The gene encoding this protein is a homolog of the bacterial pspA gene that is regulated in response to environmental stress. Given its integral role in sensing a variety of membrane stressors, it is hypothesized that pspA may play an important role in hypo- and/or hypersaline adaptation in Hfx. volcanii. Planned research will address the following questions: 1) do genes encoding salinity-regulated proteins share a common mechanisms of regulation?; 2) does Hfx. volcanii PspA function as a transcriptional activator and in a manner akin to that seen in bacteria?; 3) does Hfx. volcanii induce similar types of genes/proteins in response to low-salt stress as those seen under high-salt inducing conditions? The information obtained in this research will shed critical insight into how Hfx. volcanii is able to sustain growth and remain viable over a wide range of salinities in its natural environment. Furthermore, by providing the first experimental evidence for stress-regulated PspA activity in Archaea, its functional relationship to homologs present in bacterial and eukaryotic domains of life can be elucidated. The broader impacts of this study will chiefly involve the participation and training of a number of undergraduate research students, as this research takes place at Rider University, a small, liberal arts school with no graduate programs in the sciences.

该项目旨在在研究中采用广泛的蛋白质组学，遗传和生物信息学技术，以进一步表征这些基因及其相应的功能，这对于适应模型Haloarchaeon，Haloferax dolcanii中的可变盐分所必需。蛋白质组学分析导致​​在HFX中发现了一组盐度调节的蛋白质。现在等待其调节机制的详细表征的火山。 在确定的蛋白质中，有一种细菌样转录激活剂，该激活剂受转录水平下的NaCl浓度调节。编码该蛋白的基因是细菌PSPA基因的同源物，该基因受环境应激的调节。 鉴于其在感知各种膜应激源方面的重要作用，假设PSPA可能在HFX中的低盐和/或高盐适应中起重要作用。火山。 计划的研究将解决以下问题：1）编码盐度调节蛋白质的基因是否具有常见的调节机制？ 2）做HFX。 Volcanii Pspa充当转录激活剂，并且以类似于细菌的方式？ 3）做HFX。火山诱导的基因/蛋白质响应低盐应激的响应与高盐诱导条件下的低盐应激？在这项研究中获得的信息将使人们对HFX的方式进行批判性洞察力。 Volcanii能够维持增长，并在其自然环境中的各种盐分中保持可行。 此外，通过提供古细菌中应力调节的PSPA活性的第一个实验证据，可以阐明其与生命的细菌和真核域中存在的同源物的功能关系。 这项研究的更广泛影响将主要涉及许多本科研究专业的学生的参与和培训，因为这项研究是在骑手大学（Rider University）进行的。