RUI: Examination of the Genetic Mechanisms Used to Control the Expression of Salinity-regulated Proteins in the Archaeon Haloferax volcanii

RUI:用于控制古细菌 Haloferax volcanii 中盐度调节蛋白表达的遗传机制的检查

基本信息

  • 批准号:
    0641243
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-01-01 至 2010-12-31
  • 项目状态:
    已结题

项目摘要

This project aims to employ a broad variety of proteomic, genetic, and bioinformatic techniques for use in studies that will further characterize those genes, and their corresponding functions, necessary for adaptation to variable salinities in the model haloarchaeon, Haloferax volcanii. Proteomic analyses have led to the discovery of a set of salinity-regulated proteins in Hfx. volcanii that now await detailed characterization of the mechanisms involved in their regulation. Among the proteins identified were a bacterial-like transcriptional activator that is regulated by NaCl concentrations at the level of transcription. The gene encoding this protein is a homolog of the bacterial pspA gene that is regulated in response to environmental stress. Given its integral role in sensing a variety of membrane stressors, it is hypothesized that pspA may play an important role in hypo- and/or hypersaline adaptation in Hfx. volcanii. Planned research will address the following questions: 1) do genes encoding salinity-regulated proteins share a common mechanisms of regulation?; 2) does Hfx. volcanii PspA function as a transcriptional activator and in a manner akin to that seen in bacteria?; 3) does Hfx. volcanii induce similar types of genes/proteins in response to low-salt stress as those seen under high-salt inducing conditions? The information obtained in this research will shed critical insight into how Hfx. volcanii is able to sustain growth and remain viable over a wide range of salinities in its natural environment. Furthermore, by providing the first experimental evidence for stress-regulated PspA activity in Archaea, its functional relationship to homologs present in bacterial and eukaryotic domains of life can be elucidated. The broader impacts of this study will chiefly involve the participation and training of a number of undergraduate research students, as this research takes place at Rider University, a small, liberal arts school with no graduate programs in the sciences.
该项目旨在采用各种蛋白质组学,遗传学和生物信息学技术,用于进一步表征这些基因及其相应功能的研究,这些基因及其相应功能是适应盐古菌Haloferax volcanii模型中可变盐度所必需的。蛋白质组学分析导致在Hfx中发现一组盐度调节蛋白。volcanii,现在等待详细描述其调控机制。 在所确定的蛋白质中,有一种细菌样转录激活因子,它在转录水平上受NaCl浓度的调节。编码这种蛋白质的基因是细菌pspA基因的同源物,该基因响应于环境胁迫而被调节。 鉴于其不可或缺的作用,在传感各种膜应激,它是假设,pspA可能发挥重要作用,在低和/或高盐适应Hfx。火山 计划中的研究将解决以下问题:1)编码盐度调节蛋白的基因是否具有共同的调节机制?2)是高频信号。volcanii PspA作为转录激活因子发挥作用,其方式类似于在细菌中看到的?3)是高频信号。volcanii诱导类似类型的基因/蛋白质在响应低盐胁迫下看到的高盐诱导条件下?在这项研究中获得的信息将揭示关键的洞察力如何Hfx。volcanii能够在其自然环境中的各种盐度下持续生长并保持活力。 此外,通过提供压力调节PspA活性的第一个实验证据,其功能的关系,在细菌和真核生物领域的生活中存在的同系物可以阐明。 这项研究的更广泛的影响将主要涉及一些本科研究生的参与和培训,因为这项研究发生在莱德大学,一个小型的文科学校,没有科学研究生课程。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Kelly Bidle其他文献

Kelly Bidle的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Kelly Bidle', 18)}}的其他基金

RUI: Characterization of Four Cryptic Circadian Gene Homologs In The Haloarchaeon Haloferax Volcanii
RUI:Haloarchaeon Haloferax Volcanii 中四个隐秘昼夜节律基因同源物的表征
  • 批准号:
    1051782
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
RUI: Characterization of Salinity Regulated Genes in the Haloarchaeon, Haloferax Volcanii
RUI:Haloarchaeon、Haloferax Volcanii 中盐度调节基因的表征
  • 批准号:
    0234137
  • 财政年份:
    2003
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
POWRE: Isolation and characterization of salt-sensitive mutants in the archaeal halophile, Haloferax volcanii
POWRE:古菌嗜盐菌 Haloferax volcanii 中盐敏感突变体的分离和表征
  • 批准号:
    9972968
  • 财政年份:
    1999
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Postdoctoral Research Fellowship in Biosciences Related to the Environment for FY 1996
1996财年环境相关生物科学博士后研究奖学金
  • 批准号:
    9627134
  • 财政年份:
    1996
  • 资助金额:
    --
  • 项目类别:
    Fellowship Award

相似海外基金

Elucidation of genetic and epigenetic control mechanisms in cancer development and establishment of clinical examination methods
阐明癌症发生过程中的遗传和表观遗传控制机制并建立临床检查方法
  • 批准号:
    19K19250
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
A quantitative examination of cellular mechanisms that modulate the impacts of genetic variation
调节遗传变异影响的细胞机制的定量检查
  • 批准号:
    10194549
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
A quantitative examination of cellular mechanisms that modulate the impacts of genetic variation
调节遗传变异影响的细胞机制的定量检查
  • 批准号:
    10002278
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
A quantitative examination of cellular mechanisms that modulate the impacts of genetic variation
调节遗传变异影响的细胞机制的定量检查
  • 批准号:
    10431832
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
A quantitative examination of cellular mechanisms that modulate the impacts of genetic variation
调节遗传变异影响的细胞机制的定量检查
  • 批准号:
    10650369
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
A quantitative examination of cellular mechanisms that modulate the impacts of genetic variation
调节遗传变异影响的细胞机制的定量检查
  • 批准号:
    10623895
  • 财政年份:
    2019
  • 资助金额:
    --
  • 项目类别:
Development of the examination of genetic polymorphisms to identify high risk patients for pressure ulcer occurence
开发基因多态性检查以识别压疮发生高危患者
  • 批准号:
    18H03068
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Integrated analysis of dementia-induced wandering using postmortem CT, pathological examination, genetic polymorphism and cerebrospinal fluid biochemistry
死后CT、病理检查、基因多态性及脑脊液生化综合分析痴呆所致游走
  • 批准号:
    18K17415
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Examination of gene expression using translating ribosome affinity purification (TRAP) in genetic models of Parkinson's disease
使用翻译核糖体亲和纯化 (TRAP) 检查帕金森病遗传模型中的基因表达
  • 批准号:
    1943542
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
    Studentship
Genetic variance in the endocannabinoid system and vulnerability towards the impact of early life adversity on emotional behavior and sensitivity to stress: An examination of gene-environment interactions relevant for psychiatric illness.
内源性大麻素系统的遗传变异以及早年逆境对情绪行为和压力敏感性影响的脆弱性:与精神疾病相关的基因-环境相互作用的检查。
  • 批准号:
    377311
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
    Studentship Programs
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了