Single-Molecule Probing of Binding to Receptors

受体结合的单分子探测

• 批准号: 0649508
• 负责人: Mary Wirth
• 金额: $ 56.5万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-15 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649508&HistoricalAwards=false
• 关键词: Single; Molecule; Probing; Binding; Receptors

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. Mary Wirth of the University of Arizona. Prof. Wirth and her students will investigate how the binding of a peptide ligand to a G-Protein Coupled Receptor (GPCR) translates to receptor conformational change. Prof. Wirth and her students will develop and employ single molecule fluorescence microscopy to realize this ambitious goal. Success of the project would lead to adaptation and acceptance of single molecule microscopy and spectroscopy as complementary tools to NMR and X-Ray crystallography in studying biological systems. In the long term increased understanding of the interactions between peptide ligands and GPCRs may lead to the development of new drugs that would target this important group of receptors. The research of Prof. Wirth will provide excellent training opportunities for students and postdoctoral research associates in a highly multidisciplinary area at the forefront of scientific research.

化学系的分析与表面化学（ASC）项目将支持亚利桑那大学Mary Wirth教授的研究项目。 Wirth 教授和她的学生将研究肽配体与 G 蛋白偶联受体 (GPCR) 的结合如何转化为受体构象变化。 Wirth 教授和她的学生将开发并使用单分子荧光显微镜来实现这一雄心勃勃的目标。 该项目的成功将导致单分子显微镜和光谱学的适应和接受，作为研究生物系统中核磁共振和 X 射线晶体学的补充工具。 从长远来看，加深对肽配体和 GPCR 之间相互作用的了解可能会导致针对这一重要受体群的新药的开发。 Wirth 教授的研究将为处于科学研究前沿的高度多学科领域的学生和博士后研究员提供极好的培训机会。