Division site selection in Bacillus subtilis: Structure and function of the novel component MinJ

枯草芽孢杆菌的分裂位点选择：新成分 MinJ 的结构和功能

• 批准号: 182460133
• 负责人: Professor Dr. Marc Bramkamp
• 金额: --
• 依托单位: Institut für Allgemeine Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/182460133?language=en
• 关键词: Division; site; selection; Bacillus; subtilis

In rod-shaped bacteria the site of septation is usually found with exquisite precision. In Escherichia coli and Bacillus subtilis two inhibitory systems have been connected with division site selection, the nucleoid occlusion system and the Min system. In B. subtilis the Min system was supposed to consist out of three proteins, DivIVA, MinD, and MinC. MinCD is the actual inhibitory complex of cytokinesis, while DivIVA imposes topological control on MinCD. We and others recently identified a new member of the B. subtilis Min system, MinJ. It came as a surprise to learn that MinJ is an actual adapter that bridges DivIVA and MinD. We have found that the role of the MinCDJ system controls cytokinesis not only at the level of FtsZ inhibition, but also at the level of membrane integral division proteins. In this proposal I wish to address five major points that will help to understand the function of the B. subtilis Min system in more detail. i) We will map the interactions sites that MinJ has with DivIVA, MinD, and with the membrane integral division proteins. ii) We want to obtain structural information of MinJ. iii) We will investigate what the exact effect of MinCD on the membrane proteins of the divisome is iv) We will employ newly developed screening techniques to answer the question whether there are hitherto unidentified partners in this process. v) We wish to find out how the inhibitory function of MinCD is overcome during sporulation of B. subtilis, although the Min system is still active under these conditions.

在杆状细菌中，通常可以精确地找到分隔的位置。在大肠杆菌和枯草芽孢杆菌中，有两个抑制系统与分裂位点选择有关，即类核封闭系统和Min系统。在枯草杆菌中，Min系统被认为由三种蛋白质组成，DivIVA、Mind和Minc。MinCD是胞质分裂的实际抑制复合体，而DivIVA对MinCD施加拓扑控制。我们和其他人最近发现了枯草杆菌Min系统的一个新成员，Minj。令人惊讶的是，Minj是连接DivIVA和Mind的真正适配器。我们发现，MinCDJ系统不仅在FtsZ抑制水平上控制细胞质分裂，而且在膜整合分裂蛋白水平上也控制细胞质分裂。在这项提案中，我希望阐述有助于更详细地了解枯草杆菌Min系统功能的五个要点。I)我们将绘制Minj与DivIVA、Mind以及与膜整合分裂蛋白的相互作用部位图。Ii)我们想要获得Minj的结构信息。Iii)我们将研究MinCD对分裂体膜蛋白的确切作用是什么。iv)我们将使用新开发的筛选技术来回答在这一过程中是否有迄今尚未确定的合作伙伴的问题。V)我们希望了解在枯草杆菌产孢过程中MinCD的抑制功能是如何被克服的，尽管Min系统在这些条件下仍然是活跃的。