Systematic Plasmid Libraries for Genetic Analysis in Yeast

用于酵母遗传分析的系统质粒文库

• 批准号: 0718464
• 负责人: Gregory Prelich
• 金额: $ 21.5万
• 依托单位: Yeshiva University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718464&HistoricalAwards=false
• 关键词: Systematic; Plasmid; Libraries; Genetic; Analysis

The development of random genomic DNA libraries was an important technological breakthrough that enabled the physical isolation of any gene. Random libraries continue to be used in genetic screens and for cloning genes, but they suffer from the problems that not all portions of the genome are present, and that not all portions are present at equal levels. The goal of this project is to overcome these problems by constructing systematic libraries of the Saccharomyces cerevisiae genome. Significant progress has been made towards this goal, but physical and functional gaps remain. These gaps will be filled, and the inserts from the entire library will be transferred to another vector, creating a matching pair of systematic libraries for low level expression and for overexpression of every yeast gene. The resulting libraries will enable efficient and comprehensive overexpression screens and facilitate cloning of genes by plasmid complementation.

随机基因组DNA文库的开发是一项重要的技术突破，使任何基因的物理分离成为可能。 随机文库继续用于遗传筛选和克隆基因，但它们存在的问题是，并非基因组的所有部分都存在，并且并非所有部分都以相等的水平存在。该项目的目标是通过构建酿酒酵母基因组的系统文库来克服这些问题。在实现这一目标方面取得了重大进展，但在物质和功能方面仍然存在差距。 这些缺口将被填补，并且来自整个文库的插入片段将被转移到另一个载体中，从而为每个酵母基因的低水平表达和过表达创建匹配的系统文库对。 由此产生的文库将能够进行有效和全面的过表达筛选，并通过质粒互补促进基因的克隆。