Biological and pathological characterisation of novel plasmid-carrying avian Chlamydia abortus strain 84/2334

新型质粒携带禽流产衣原体菌株 84/2334 的生物学和病理学特征

基本信息

  • 批准号:
    BB/X016692/1
  • 负责人:
  • 金额:
    $ 131.57万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2023
  • 资助国家:
    英国
  • 起止时间:
    2023 至 无数据
  • 项目状态:
    未结题

项目摘要

Many infectious diseases affect livestock, impacting not only on the health and welfare of the animals but also on the economic sustainability of the agricultural sector and future food security. Ovine enzootic abortion (OEA) is an economically important endemic disease of worldwide significance in small ruminant species, caused by the Gram-negative bacterium Chlamydia abortus. In the UK it is the most common cause of infectious abortion in sheep, resulting in foetal death in the last 2-3 weeks of pregnancy or the delivery of weak offspring and causing losses in a flock of up to around 30%. Losses in the UK have been estimated to be around £25M per annum. The organism can also infect humans causing spontaneous abortion, and in whom infections can be life-threatening. Vaccination is currently considered the most effective way of controlling disease and vaccines based on inactivated or live whole-organisms are commercially available for use in small ruminants. However, live vaccines have been reported to cause infection and disease in some animals and inactivated vaccines have been found to have much lower effectiveness in protecting animals from infections. Thus, there is a need to develop safer, more effective vaccines for use in livestock. Reducing infection and disease burden in animals will also reduce the risk of infections in humans. In recent years, research has progressed into developing methods for manipulating the genomes of human chlamydial species, making use of an endogenous plasmid (DNA that is extra to that present on the chromosome) that many of the strains possess. These plasmids are also present in avian chlamydial species (Chlamydia psittaci) from which C. abortus evolved. However, no plasmid has been found to date in C. abortus strains. Recently, we have characterised the genome of a novel strain, designated 84/2334, which has been reclassified from C. psittaci to C. abortus and carries a plasmid. Therefore, this strain could potentially be used to develop a new C. abortus vaccine for controlling OEA. However, before doing this, we need to know how biologically similar the strain is to C. abortus and determine whether the strain causes infection and disease similar to that caused by C. abortus. In this project we will compare the growth dynamics of the 84/2334 strain in relation to both C. abortus and C. psittaci, investigate its growth cycle in the target host cell, identify the key molecules involved in causing pathogenesis, and determine its potential to cause infection and abortion. These studies will identify whether the strain is more similar to classical C. abortus than to C. psittaci and determine its suitability for use in future vaccine development studies.
许多传染病影响到牲畜,不仅影响到牲畜的健康和福利,而且影响到农业部门的经济可持续性和未来的粮食安全。绵羊地方性流产(OEA)是由革兰氏阴性菌流产衣原体(Chlamydia abortus)引起的一种世界性的地方性疾病。在英国,它是绵羊感染性流产的最常见原因,导致妊娠最后2-3周的胎儿死亡或分娩虚弱的后代,并造成高达30%左右的羊群损失。英国的损失估计每年约为2500万英镑。该生物体也可以感染人类,导致自然流产,感染可能危及生命。疫苗接种目前被认为是控制疾病的最有效方法,基于灭活或活的全生物体的疫苗可商购获得,用于小型反刍动物。然而,据报道,活疫苗会在一些动物中引起感染和疾病,而灭活疫苗在保护动物免受感染方面的效力要低得多。因此,需要开发更安全、更有效的疫苗用于牲畜。减少动物的感染和疾病负担也将降低人类感染的风险。近年来,研究已经进展到开发用于操纵人类衣原体物种的基因组的方法,利用许多菌株所具有的内源性质粒(DNA,其是存在于染色体上的DNA之外的)。这些质粒也存在于禽类衣原体种(鹦鹉热衣原体)中,其中C. abortus进化了。然而,迄今为止在C.流产菌株。最近,我们已经鉴定了一种新菌株的基因组,命名为84/2334,该菌株已从C. psittaci到C.流产并携带质粒。因此,该菌株有可能用于开发新的C.用于控制OEA的流产疫苗。然而,在这样做之前,我们需要知道该菌株与C的生物相似性如何。并确定该菌株是否引起与C.流产在这个项目中,我们将比较84/2334菌株与C. abortus和C.鹦鹉热,研究其在靶宿主细胞中的生长周期,确定参与引起发病机制的关键分子,并确定其引起感染和流产的潜力。这些研究将确定该菌株是否与经典C. abortus比C.鹦鹉热,并确定其适用于未来的疫苗开发研究。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

David Longbottom其他文献

Subunit structure of calgranulins A and B obtained from sputum, plasma, granulocytes and cultured epithelial cells
  • DOI:
    10.1016/0167-4838(92)90273-g
  • 发表时间:
    1992-04-08
  • 期刊:
  • 影响因子:
  • 作者:
    David Longbottom;Jean-Michel Sallenave;Veronica van Heyningen
  • 通讯作者:
    Veronica van Heyningen
Edinburgh Research Explorer Presence of DNA from Chlamydia-like organisms in the nasal cavities of grey seal pups (Halichoerus grypus) and three different substrates present in a breeding colony
爱丁堡研究探索者灰海豹幼崽(Halichoerus grypus)鼻腔中存在类衣原体生物的 DNA 以及繁殖群体中存在的三种不同底物
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M. P. Dagleish;A. Flockhart;J. Baily;Ailsa J. Hall;T. Simpson;David Longbottom
  • 通讯作者:
    David Longbottom
Single channel analysis of recombinant major outer membrane protein porins from Chlamydia psittaci and Chlamydia pneumoniae
鹦鹉热衣原体和肺炎衣原体重组主要外膜蛋白孔蛋白的单通道分析
  • DOI:
    10.1016/s0014-5793(99)00121-0
  • 发表时间:
    1999
  • 期刊:
  • 影响因子:
    3.5
  • 作者:
    Susan Wyllie;David Longbottom;A. J. Herring;Richard H. Ashley
  • 通讯作者:
    Richard H. Ashley
Identification of a multigene family coding for the 90 kDa proteins of the ovine abortion subtype of Chlamydia psittaci.
编码鹦鹉热衣原体绵羊流产亚型 90 kDa 蛋白的多基因家族的鉴定。
  • DOI:
  • 发表时间:
    1996
  • 期刊:
  • 影响因子:
    2.1
  • 作者:
    David Longbottom;Mary Russell;Gareth E. Jones;F. Lainson;A. J. Herring
  • 通讯作者:
    A. J. Herring
Inflammatory cytokine responses in a pregnant mouse model of <em>Chlamydophila abortus</em> infection
  • DOI:
    10.1016/j.vetmic.2010.01.025
  • 发表时间:
    2010-08-26
  • 期刊:
  • 影响因子:
  • 作者:
    Karen Kerr;Nicholas Wheelhouse;Morag Livingstone;Ian E. Anderson;Gary Entrican;Declan McKeever;David Longbottom
  • 通讯作者:
    David Longbottom

David Longbottom的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('David Longbottom', 18)}}的其他基金

Emerging Chlamydia-like organisms as novel causes of bovine reproductive failure
新兴的衣原体类生物体是牛繁殖失败的新原因
  • 批准号:
    BB/J015083/1
  • 财政年份:
    2013
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Research Grant
Integrated genomic and proteomic characterisation of autotransporter proteins of obligate intracellular bacteria C. abortus and L. intracellularis
专性胞内细菌 C. abortus 和 L. intracellularis 的自转运蛋白的综合基因组和蛋白质组学特征
  • 批准号:
    BB/E018939/1
  • 财政年份:
    2008
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Research Grant

相似国自然基金

生理/病理应激差异化调控肝再生的“蓝斑—中缝”神经环路机制
  • 批准号:
    82371517
  • 批准年份:
    2023
  • 资助金额:
    49.00 万元
  • 项目类别:
    面上项目
直肠癌术前同期放化疗后pCR病例的筛选模型研究
  • 批准号:
    81071891
  • 批准年份:
    2010
  • 资助金额:
    10.0 万元
  • 项目类别:
    面上项目

相似海外基金

EAGER: Integrating Pathological Image and Biomedical Text Data for Clinical Outcome Prediction
EAGER:整合病理图像和生物医学文本数据进行临床结果预测
  • 批准号:
    2412195
  • 财政年份:
    2024
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Standard Grant
Transient Reset Of The Pathological Brain
病理性大脑的短暂重置
  • 批准号:
    EP/Z000165/1
  • 财政年份:
    2024
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Research Grant
International Centre of Excellence in the Treatment of Pathological Fractures
国际病理性骨折治疗卓越中心
  • 批准号:
    EP/Z531248/1
  • 财政年份:
    2024
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Research Grant
Neural substrates of extinction deficits in pathological fear
病理性恐惧中消退缺陷的神经基础
  • 批准号:
    10999104
  • 财政年份:
    2024
  • 资助金额:
    $ 131.57万
  • 项目类别:
Systematic manipulation of tau protein aggregation: bridging biochemical and pathological properties
tau 蛋白聚集的系统操作:桥接生化和病理特性
  • 批准号:
    479334
  • 财政年份:
    2023
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Operating Grants
Pathological analysis using transgenic rats that produce acute retinitis pigmentosa.
使用产生急性色素性视网膜炎的转基因大鼠进行病理分析。
  • 批准号:
    23K09034
  • 财政年份:
    2023
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathological study of muscle fiber atrophy in aged persons or alcoholics
老年人或酗酒者肌纤维萎缩的病理学研究
  • 批准号:
    23K09768
  • 财政年份:
    2023
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional and pathological analysis of monocytes and macrophages in toxic epidermal necrolysis
中毒性表皮坏死松解症中单核细胞和巨噬细胞的功能和病理分析
  • 批准号:
    23K07788
  • 财政年份:
    2023
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of BET proteins in pathological cardiac remodeling
BET蛋白在病理性心脏重塑中的作用
  • 批准号:
    10538142
  • 财政年份:
    2023
  • 资助金额:
    $ 131.57万
  • 项目类别:
In vivo and ex vivo lessons from somatic adrenal mutations in cell adhesion molecule 1 for physiological and pathological production of aldosterone
细胞粘附分子 1 体细胞肾上腺突变对醛固酮生理和病理产生的体内和离体教训
  • 批准号:
    MR/X018970/1
  • 财政年份:
    2023
  • 资助金额:
    $ 131.57万
  • 项目类别:
    Fellowship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了