RUI: Model Compounds for P460 from Hydroxylamine Oxidoreductase

RUI：羟胺氧化还原酶 P460 的模型化合物

• 批准号: 0718749
• 负责人: Edward Zovinka
• 金额: $ 9.5万
• 依托单位: Saint Francis University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718749&HistoricalAwards=false
• 关键词: RUI; Model; Compounds; P460; Hydroxylamine

Professor Edward P. Zovinka, Department of Chemistry, Mathematics, and Physical Science, Saint Francis University is supported by the Inorganic, Bioinorganic and Organometallic Chemistry Program to study models for hydroxylamine oxidoreductase (HAO) in Nitrosomonas europea (Ne), an autotrophic soil bacteria that oxidizes nitrogenous materials in order to make energy. HAO contains a unique tyrosine-porphyrin connection that has been speculated to play an important role in the enzyme''s activity. To better understand the chemistry of the active site, undergraduate researchers synthesize and study model compounds containing meso-(hydroxyphenyl)porphyrin ligands as analogs for the porphyrin-tyrosine moiety. The porphyrin ligands are coordinated to biologically significant iron and the reactivities of the compounds with hydroxylamine are studied. The group utilizes Green Chemistry principles in the synthesis of the ligands. This includes the use of microwave methods to improve reaction yields and decrease reaction times. Scientific ethics are stressed to all participating undergraduate researchers through literature discussions.

圣弗朗西斯大学化学、数学和物理科学系爱德华·P·佐文卡教授在无机化学、生物无机化学和有机金属化学计划的支持下，研究欧洲亚硝酸单胞菌(NitroSomonas Europe a，Ne)中羟胺氧化还原酶(HAO)的模型。HOO含有一种独特的酪氨酸-卟啉连接，被认为在酶S的活性中起着重要作用。为了更好地了解活性部位的化学，本科生研究人员合成并研究了含有中-羟基苯基卟啉配体的模型化合物，作为卟啉-酪氨酸部分的类似物。卟啉配体与具有生物意义的铁配位，并研究了化合物与羟胺的反应活性。该小组在配体的合成中利用绿色化学原理。这包括使用微波方法来提高反应产率和减少反应时间。通过文献讨论，所有参与的本科生研究人员都强调了科学伦理。