Development of a Fourier Transform Tip - Enhanced Coherent Anti-Stokes Raman Scattering Microscope

在化学研究仪器和设施仪器开发项目的支持下，密歇根大学化学系的Jennifer Ogilvie博士提议开发一种傅立叶变换尖端增强相干反斯托克斯拉曼散射显微镜（FT-TECARS）。该仪器将以拉曼光谱的形式提供样品的化学详细表征，亚衍射极限分辨率为~ 20 nm。该方案旨在结合原子力显微镜的扫描尖端来增强CARS信号，结合迈克尔逊干涉仪来实现高光谱分辨率，结合纯差检测来增强对远场CARS背景干扰的辨别能力。傅里叶变换的实现，结合飞秒激光的宽带激发，将允许在指纹区域收集数据。有许多研究项目可能受益于这种仪器，因为它将允许人们深入了解空间微小表面结构的键合和化学性质。Ogilvie博士已经决定FT-TECARS仪器的初始项目是研究由磷脂/胆固醇混合物组成的模型膜的相行为。

With support from the Chemistry Research Instrumentation and Facilities Instrument Development program, Dr. Jennifer Ogilvie of the Department of Chemistry of the University of Michigan proposes to develop a Fourier transform tip-enhanced coherent anti-Stokes Raman scattering microscope (FT-TECARS). This instrument will provide chemically detailed characterization of samples, in the form of Raman spectra, with sub-diffraction-limited resolution of ~ 20 nm. The proposal aims to combine the scanning tip of an atomic force microscope to enhance the CARS signal, with a Michelson interferrometer to achieve high spectral resolution, and homodyne detection to increase discrimination against interference from far-field CARS background. The Fourier transform implementation, combined with the femtosecond laser for broad band excitation, will permit data collection over the finger print region. There are a number of research projects that could potentially benefit from such an instrument since it would allow one to gain insight into bonding and chemical properties of spatially minute surface structures. Dr. Ogilvie has decided the initial project for the FT-TECARS instrument to be the study phase behavior of model membranes composed of phospholipid/cholesterol mixtures.