权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Microbial life under extreme CO2 exhalations

极端二氧化碳排放下的微生物生命

基本信息

批准号：
183403997
负责人：
Professorin Dr. Kirsten Küsel
金额：
--
依托单位：
Lehrstuhl Aquatische Geomikrobiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/183403997?language=en
关键词：
Microbial life under extreme CO2

项目摘要

In mofettes, geogenic CO2 is emitted in extremely high concentrations to the atmosphere leading to soil acidification and anoxic conditions. The microbial community colonizing the core zone of mofettes has to be able to tolerate these extremes and might be distinct from unaffected terrestrial reference sites. Since capture of CO2 in geological reservoirs is discussed as promising disposal strategy, mofettes might be an ideal site to study potential ecological impacts of CO2 storage and leakage on biogeochemical processes and microbial communities. Thus, this project will unravel the diversity and biogeochemistry of the microbial community inhabiting a mofette located in the Plesna valley (NW-Czechia). The assimilation of CO2 and its use as terminal electron acceptor in the absence of oxygen should be significant for microbial processes in mofettes. The composition of 13C in pore water and soil gas compounds linked with 13C depletion of specific archaeal and bacterial lipids will give further insights into the dominance of microbial populations adapted to exploiting geogenic CO2. Since CH4 is not emitted at most mofette sites, CO2 might be reduced to acetate by acetogenic bacteria which will be quantified based on their functional fhs marker gene. Their metabolic versatility and oxygen tolerance might enable acetogens to colonize both anoxic soils with extremely high CO2 concentrations and also the rhizosphere of plants, like Eriophorum vaginatum, that appears to be adapted to CO2-rich soils by the transport of O2 to their roots. For enrichment and potential isolation of novel “moffetophilic” microorganisms, the gradient tube technique will be used and adjusted to the in situ conditions.

在mofettes中，地质二氧化碳以极高的浓度排放到大气中，导致土壤酸化和缺氧条件。定居在莫费特核心区的微生物群落必须能够忍受这些极端情况，并且可能与未受影响的陆地参考地点不同。由于捕获的CO2在地质水库被认为是有前途的处置策略，mofettes可能是一个理想的网站，研究潜在的生态影响的CO2储存和泄漏的生态地球化学过程和微生物群落。因此，该项目将揭示居住在Plesna山谷（西北-捷克）的微生物群落的多样性和生物地球化学。CO2的同化作用及其在无氧条件下作为末端电子受体的作用对毛状体中的微生物过程具有重要意义。孔隙水和土壤气体化合物中的13 C组成与特定古细菌和细菌脂质的13 C消耗有关，这将进一步深入了解适应于利用地质CO2的微生物种群的优势。由于大多数mofette站点不排放CH 4，因此CO2可能被产乙酸细菌还原为乙酸盐，这将基于其功能性fhs标记基因进行定量。他们的代谢的多功能性和耐氧性可能使产乙酸菌殖民缺氧土壤具有极高的CO2浓度和根际的植物，如Eriophorum vaginatum，这似乎是适应富CO2土壤的O2运输到他们的根。为了富集和潜在分离新的“嗜咖啡因”微生物，将使用梯度管技术并调整至原位条件。