Nitric Oxide Transport Mechanisms: An Engineering approach

[07:30547 . 47]该提案涉及开发一种多尺度方法来模拟一氧化氮的产生、运输和代谢。该提案共有5个目标：(1)扩展现有的细胞尺度模型，包括细胞内信号传导和细胞外运输；(2)将血管尺度和细胞尺度模型结合起来；(3)开发血管网络尺度的NO运输模型；(4)将模型扩展到组织水平；(5)通过体外和体内研究验证理论模拟。一氧化氮的转运和代谢在许多正常和病理组织功能中起着重要作用。因此，本研究将对医学和血管生理学做出重要贡献。在一个集成的多尺度模型中，血流动力学、多种化学反应和生物物理过程的耦合对大量重要的生理和病理生理过程至关重要。建议的研究与现有课程和其他教育组成部分的耦合是本建议的优势。特别是，包括细胞生物力学、组织工程和心血管工程课程在内的这项工作将给学生一个学习一些前沿研究的机会。这些私人学院在他们的研究项目中有包括本科生的记录。此外，多尺度和多时间方法在生物学和生理学中也变得越来越重要。因此，除了NO代谢，这项工作还可能影响许多其他领域。

0730547JaronThis proposal concerns the development a multi-scale approach to modeling nitric oxide production, transport and metabolism. A total of 5 objectives are stated for this proposal: (1) Expand an existing cell-scale model to include intracellular signaling and extra-cellular transport, (2) Couple a vascular-scale and cellular-scale model, (3) Develop a vascular network-scale model of NO transport, (4) Extend the model to a tissue level scale, and (5) Validate the theoretical simulations with in vitro and in vivo studies. The transport and metabolism of NO plays an important role in many normal and pathological tissue functions. As a result, this study could make important contributions to medicine and vascular physiology in general. The coupling of hemodynamics, multiple chemical reactions, and biophysical processes in an integrated multi-scale model is central to a vast number of important physiological and pathophysiological processes.The coupling of the proposed research with existing courses and other education components is a strength of this proposal. In particular, including this work in cellular biomechanics, tissue engineering, and cardiovascular engineering course work will give students an opportunity to learn some cutting edge research. The PIs have a track record of including undergraduates in their research programs. Also, multi-scale and multi-temporal methods are becoming more important in biology and physiology. As a result, this work is likely to impact a number of other areas besides NO metabolism.