Project laboratory in genetics and genomics

遗传学和基因组学项目实验室

• 批准号: 0736995
• 负责人: Susan Lovett
• 金额: $ 15万
• 依托单位: Brandeis University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0736995&HistoricalAwards=false
• 关键词: Project; laboratory; genetics; genomics

Biological Sciences (61)In the laboratory course being developed by this project students combine genetics and genomics techniques to explore genetic variation within populations. It is based on a pilot course introduced in 2007. Students isolate random E. coli transposon mutations affecting rates of genetic variation and analyze the unique mutants they have isolated to discover and understand functions essential to genetic stability. They then integrate their findings with public domain genomic information resources to develop a Web page for each gene investigated. As a finale for the course, students design their own simple experiment regarding mutagenesis and refine the experiment from the results of their preliminary analysis. Students are assessed, before and after the course, for their level of mastery of basic cellular and molecular processes and for their attitudes towards and understanding of scientific research. In addition, students evaluate the value of various aspects of the course, to aid in its future refinements. The intellectual merit of this project is that it provides real research laboratory experience in a course that leads to understanding of core concepts in genetics.The broader impact of this project is that the course serves as a model for future development of interdisciplinary project laboratories at Brandeis University and elsewhere. Course materials (information, protocols, genomic resources, exercises, design, strains) are being made available publicly. The novel integration of genomic analysis with readily accessible experiments with bacteria provides a course paradigm that can be replicated in diverse academic settings.

生物科学（61）在本项目开发的实验室课程中，学生将联合收割机遗传学和基因组学技术结合起来，探索种群内的遗传变异。 该课程以2007年推出的试点课程为基础。学生们随机分离E.大肠杆菌转座子突变影响遗传变异率，并分析他们分离的独特突变体，以发现和理解遗传稳定性所必需的功能。然后，他们将他们的发现与公共领域基因组信息资源整合，为每个研究的基因开发一个网页。作为本课程的最后一课，学生设计自己关于诱变的简单实验，并根据初步分析的结果改进实验。在课程之前和之后，对学生进行评估，以了解他们对基本细胞和分子过程的掌握程度，以及他们对科学研究的态度和理解。此外，学生评估课程的各个方面的价值，以帮助其未来的改进。这个项目的智力价值在于它提供了真实的研究实验室经验，这门课程导致了对遗传学核心概念的理解。这个项目的更广泛的影响是，这门课程成为布兰代斯大学和其他地方跨学科项目实验室未来发展的典范。 课程材料（信息、方案、基因组资源、练习、设计、菌株）正在公开提供。基因组分析与易于获得的细菌实验的新颖整合提供了一种可以在不同学术环境中复制的课程范式。