SBIR Phase I: Chemoenzymatic Production of Innovative Sesquiterpene-Based Fragrance Libraries

这个小企业创新研究（SBIR）第一阶段研究项目旨在开发一个基于酵母的平台，用于产生新型倍半萜化合物，以及商业上不可用的倍半萜支架。 该技术结合了代谢、蛋白质和生物化学方法来产生限定的倍半萜烯支架，这些倍半萜烯支架又用于使用组合化学来产生感兴趣的化合物的文库。 所产生的化合物对香料工业具有商业价值，但也可以筛选与医学相关的性质。 甲羟戊酸和非甲羟戊酸途径是目前已知用于合成异戊烯焦磷酸的唯一途径，异戊烯焦磷酸是类异戊二烯（萜烯）的前体之一，类异戊二烯是结构上最多样化的一类生物化合物。 这些前体通过一系列酶促反应转化为萜烯。 该技术旨在利用对萜烯生物化学和酶学的理解，开发一种能够生产数千种具有有趣特性和巨大商业价值的新倍半萜的工艺。

This Small Business Innovation Research (SBIR) Phase I research project aims to develop a yeast-based platform for the generation of novel sesquiterpene compounds, as well as commercially unavailable sesquiterpene scaffolds. The technique combines metabolic, protein and biochemical approaches to produce defined sesquiterpene scaffolds, which are in turn, used to produce libraries of interesting compounds using combinatorial chemistry. The generated compounds are commercially valuable to the fragrance industry, but could also be screened for medically relevant properties. The mevalonate and non-mevalonate pathways are currently the only ones known to be used for the synthesis of isopentenyl pyrophosphate, one of the precursors to isoprenoids (terpenes), the most structurally diverse class of biological compounds. These precursors are converted to the terpenes through a series of enzymatic reactions. The proposed technology aims to leverage the understanding of the biochemistry and enzymology of the terpenes to develop a process that enables the production of thousands of new sesqueterpenes with interesting properties and great commercial value.