CAREER: Heterogeneous Cell Carriers to Promote Gradiated Tissue Formation Under Mechanical Loading - An Integrated Education and Research Study

不同组织类型之间的界面遍布全身，对所有主要器官系统的正常功能至关重要。然而，在许多情况下，仍然不清楚这些界面是如何形成的，这使得在损伤后重建不同组织之间的适当相互作用变得非常困难。该项目的预期成果是将开发一种可控的三维共培养系统作为骨科组织界面发育的模型。这个项目是重要的，因为模型系统将提供关键信息的作用，促进梯度的表型表达的间充质干细胞（MSC）在拉伸负荷下，如在骨韧带界面在体内经历的细胞-基质相互作用。该教育项目包括在高中，大学和研究生阶段创建新的和创新的教学材料，这将促进对确保现代生物材料安全所需的复杂过程的理解和欣赏，并突出当前生物材料的局限性。

0746209TemenoffAbstractInterfaces between different tissue types are found throughout the body and are crucial to the proper functions of all major organ systems. However, in many cases, it remains unclear how these interfaces form, making it extremely difficult to recreate appropriate interactions between disparate tissues after injury. An anticipated outcome of this project is that a controlled, three-dimensional coculture system as a model for interface development in orthopaedic tissues will be developed. This project is significant because the model system will provide key information about the role of cell-matrix interactions in promoting a gradient of phenotypic expression by mesenchymel stem cells (MSCs) under tensile loading, such as that experienced at the bone-ligament interface in vivo. The education project includes the creation of new and innovative teaching materials at the high school, college, and graduate levels that will advance understanding and appreciation of the complex processes required to assure safety of modern biomaterials, and highlight limitations of current biomaterials.