CAREER:Transfected cell microarray technology based on microfluidic electroporation

这个项目的广泛和长期的目标是开发基于微流控的转基因细胞阵列，使用电穿孔作为基于细胞系和原代细胞的大规模基因筛选的转染方法。当达到一个临界电场强度并且细胞膜对大的生物分子具有有效的渗透性时，就会发生电穿孔。作为一种物理方法，电穿孔对细胞类型的依赖性不强，已被证明是有效的原代细胞转染法。在这个项目中，首席调查员(PI)将基于其实验室开发的新型微流控电穿孔技术，系统地研究细胞系和原代细胞的转染，并展示用于基因筛选的概念验证转基因细胞阵列。在教育计划中，PI将为初中生/高中生开发“电子实地考察”，以传播生物工程知识，并为K-12外展建立网络基础设施。国际生物工程研究所还将开发一门关于生物工程新兴技术的新课程，并在这一高度跨学科的项目中培训本科生和研究生。

0747105LuThe broad and long-term goal of this project is to develop microfluidics-based transfected cell arrays using electroporation as the transfection method for large-scale gene screening based on both cell lines and primary cells. Electroporation occurs when a critical electrical field strength has been achieved and the cell membrane becomes effectively permeable to large biomolecules. As a physical method, electroporation does not have strong cell type dependency and has been shown effective in transfecting primary cells. In this project, the Principal Investigator (PI) will systematically study the transfection of cell lines and primary cells based on a novel microfluidic electroporation technique developed in the PI's laboratory and also demonstrate proof-of-concept transfected cell arrays for gene screening. In the educational plan, the PI will develop "electronic field trips" for middle/high school students to disseminate the knowledge about biological engineering and establish cyberinfrastructure for K-12 outreach. The PI will also develop a new course on emerging technologies in biological engineering and train undergraduate and graduate students in this highly interdisciplinary project.