CAREER:Transfected cell microarray technology based on microfluidic electroporation

0747105 Lu该项目的广泛和长期目标是开发基于微流体的转染细胞阵列，使用电穿孔作为转染方法，用于基于细胞系和原代细胞的大规模基因筛选。当达到临界电场强度并且细胞膜变得对大生物分子有效可渗透时，发生电穿孔。作为一种物理方法，电穿孔不具有强烈的细胞类型依赖性，并且已经显示出在分离原代细胞中是有效的。在该项目中，主要研究者（PI）将系统地研究基于PI实验室开发的新型微流体电穿孔技术的细胞系和原代细胞的转染，并展示用于基因筛选的概念验证转染细胞阵列。在教育计划中，PI将为初中/高中学生开发“电子实地考察”，以传播有关生物工程的知识，并为K-12推广建立网络基础设施。PI还将开发一门关于生物工程新兴技术的新课程，并在这个高度跨学科的项目中培养本科生和研究生。

0747105LuThe broad and long-term goal of this project is to develop microfluidics-based transfected cell arrays using electroporation as the transfection method for large-scale gene screening based on both cell lines and primary cells. Electroporation occurs when a critical electrical field strength has been achieved and the cell membrane becomes effectively permeable to large biomolecules. As a physical method, electroporation does not have strong cell type dependency and has been shown effective in transfecting primary cells. In this project, the Principal Investigator (PI) will systematically study the transfection of cell lines and primary cells based on a novel microfluidic electroporation technique developed in the PI's laboratory and also demonstrate proof-of-concept transfected cell arrays for gene screening. In the educational plan, the PI will develop "electronic field trips" for middle/high school students to disseminate the knowledge about biological engineering and establish cyberinfrastructure for K-12 outreach. The PI will also develop a new course on emerging technologies in biological engineering and train undergraduate and graduate students in this highly interdisciplinary project.