CAREER:Transfected cell microarray technology based on microfluidic electroporation

[07747105]本项目的长远目标是开发基于微流体的转染细胞阵列，使用电穿孔作为转染方法，在细胞系和原代细胞中进行大规模基因筛选。当达到临界电场强度时，细胞膜就会有效地渗透到大分子中。电穿孔作为一种物理方法，对细胞类型依赖性不强，在转染原代细胞中已被证明是有效的。在这个项目中，首席研究员（PI）将系统地研究基于PI实验室开发的新型微流体电穿孔技术的细胞系和原代细胞的转染，并演示用于基因筛选的转染细胞阵列的概念验证。在教育计划中，PI将为初高中学生开发“电子实地考察”，以传播生物工程知识，并为K-12扩展建立网络基础设施。PI还将开发一门关于生物工程新兴技术的新课程，并在这个高度跨学科的项目中培养本科生和研究生。

0747105LuThe broad and long-term goal of this project is to develop microfluidics-based transfected cell arrays using electroporation as the transfection method for large-scale gene screening based on both cell lines and primary cells. Electroporation occurs when a critical electrical field strength has been achieved and the cell membrane becomes effectively permeable to large biomolecules. As a physical method, electroporation does not have strong cell type dependency and has been shown effective in transfecting primary cells. In this project, the Principal Investigator (PI) will systematically study the transfection of cell lines and primary cells based on a novel microfluidic electroporation technique developed in the PI's laboratory and also demonstrate proof-of-concept transfected cell arrays for gene screening. In the educational plan, the PI will develop "electronic field trips" for middle/high school students to disseminate the knowledge about biological engineering and establish cyberinfrastructure for K-12 outreach. The PI will also develop a new course on emerging technologies in biological engineering and train undergraduate and graduate students in this highly interdisciplinary project.