CAREER: A Dual Reaction Cell, ETD-enabled Orbitrap Mass Spectrometer for Top-down Proteomics

分析和表面化学计划的职业奖支持威斯康星州-麦迪逊大学的约书亚库恩教授的工作，以开发新技术来表征活细胞内产生的基因产物的许多亚型和修饰版本。 自上而下的蛋白质组学技术的适应将在小于~2秒/蛋白质的时间尺度上提供整个蛋白质的快速、稳健的表征。 一种新的电子转移解离（ETD）启用线性离子阱轨道阱质谱仪最近在库恩实验室建设将增强一个新的高容量线性RF四极杆，这将作为一个专用的离子/离子反应细胞，以允许所需的快速分析，使常规耦合的自上而下的蛋白质组学测量的蛋白质高达至少70 kDa的色谱分离。 该计划还需要开发新型的K-12教学法，以吸引学术和非学术学生的形式展示设计（技术）和探究（科学）之间的协同作用。 该计划（“TESci”）结合了不同的学生和教育工作者群体，通过跨学科的团队学习环境将技术教育（TE）和科学紧密联系起来。 该计划是跨领域的，旨在：（1）培养那些最不可能从事科学研究的人对科学的兴趣，（2）在大学理科学生中培养技术技能，（3）灌输跨学科合作和科学多样性的价值。 TESci计划将为科学和技术教育的整合创造一个卓越的中心-一个将为威斯康星州和国家提供模式的中心。

This CAREER award by the Analytical and Surface Chemistry program supports work by Professor Joshua Coon at the University of Wisconsin - Madison to develop new technologies to characterize the many isoforms and modified versions of gene products generated within living cells. Adaptation of top-down proteomic technologies will provide rapid, robust characterization of whole proteins on a time-scale of less than ~2 seconds/protein. A novel electron transfer dissociation (ETD) enabled linear ion trap-orbitrap mass spectrometer recently constructed in the Coon laboratory will be enhanced by a new high capacity linear RF quadrupole, which will serve as a dedicated ion/ion reaction cell to allow the needed rapid analyses, enabling routine coupling of top-down proteomic measurements for proteins up to at least 70 kDa with chromatographic separations. The program also entails development of novel K-12 pedagogy that demonstrates the synergy between design (technology) and inquiry (science) in a format that engages both academic and non-academic students. This program ("TESci") combines a diverse group of students and educators to tightly link technology education (TE) and the sciences through an interdisciplinary, team-learning environment. The program is cross-cutting and aims to: (1) foster an interest in the sciences among those least likely to become engaged in scientific research, (2) develop technology skills among college-bound science students, and (3) instill the value of interdisciplinary collaboration and diversity in science. The TESci program will create a hub of excellence for the integration of science and technology education - one that will provide a model for Wisconsin and the nation.