Immunosensors for water column and 2-D sediment distributions of vitamin B-12 and target organic solutes

用于维生素 B-12 和目标有机溶质的水柱和二维沉积物分布的免疫传感器

• 批准号: 0752105
• 负责人: Qingzhi Zhu
• 金额: $ 67.22万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0752105&HistoricalAwards=false
• 关键词: Immunosensors; water; column; sediment; distributions

The PI's request funding to develop ELISA methods for determination of vitamin B12 in coastal seawater and sediment pore water using both steady and time-resolved fluorescence technologies. A flow injection immunosensor based on immunoaffinity solid-phase extraction will allow rapid in-situ measurement of ultra trace B12 in seawater either by remote control technology, or on board ship. A new fluorescence resonance energy transfer (FRET) planar immunosensor, optimized based on the overlying water methodology, will be utilized for in-situ mapping of 2-dimensional vitamin B12 distribution and transport patterns in bottom deposits. Vitamin B12 is a microbially produced compound specifically required by phytoplankton for primary production in aquatic systems. It is representative of multiple trace organometallic compounds essential for biological growth and functioning, and has major sources from heterotrophic microbial communities in sediments. Its importance in phytoplankton ecology is just beginning to be understood, and the distribution, transport and factors controlling production patterns of B12 and other biogenic organometallics in marine sediments are essentially undocumented due to lack of suitable analytical tools. The capability for rapid and accurate quantitative or qualitative reconnaissance of vitamin B12 in situ would significantly advance knowledge of biogeochemical cycling of vitamins and related compounds, further reveal the influence of B12 on primary productivity and phytoplankton growth, and provide an enhanced basis for conceptual models and practical management decisions related to nutrient cycling in coastal environments. The PI?s request funding to develop ELISA methods for determination of vitamin B12 in coastal seawater and sediment pore water using both steady and time-resolved fluorescence technologies. A flow injection immunosensor based on immunoaffinity solid-phase extraction will allow rapid in-situ measurement of ultra trace B12 in seawater either by remote control technology, or on board ship. A new fluorescence resonance energy transfer (FRET) planar immunosensor, optimized based on the overlying water methodology, will be utilized for in-situ mapping of 2-dimensional vitamin B12 distribution and transport patterns in bottom deposits. Broader Impacts: The proposed work could revolutionize the way we analyze small biomolecules, and may have important applications in fields outside of chemical oceanography, like chemical ecology. This project has the potential develop a novel sensor that if successful would contribute significantly to our understanding of the influence of B12 in marine systems. These novel technologies (flow immunosensor, planar fluorescence) would be incorporated into graduate and undergraduate curricula, as well to local high school outreach programs to highly motivated students.

国际和平研究所要求提供资金，以开发使用稳定和时间分辨荧光技术测定沿海海水和沉积物孔隙水中维生素B12的酶联免疫吸附试验方法。基于免疫亲和固相萃取的流动注射免疫传感器将通过远程控制技术或在船上快速原位测量海水中的超痕量B12。一种新的基于上覆水方法优化的荧光共振能量转移(FRET)平面免疫传感器将被用于原位绘制二维维生素B12在海底沉积物中的分布和运输模式。维生素B12是一种微生物产生的化合物，浮游植物特别需要在水生系统中进行初级生产。它是生物生长和功能所必需的多种痕量有机金属化合物的代表，其主要来源是沉积物中的异养微生物群落。它在浮游植物生态学中的重要性才刚刚开始被了解，由于缺乏适当的分析工具，B12和其他生物成因金属在海洋沉积物中的分布、运输和控制生产模式的因素基本上没有文献记载。对维生素B12进行快速、准确的定量或定性现场调查的能力将大大提高对维生素和相关化合物的生物地球化学循环的认识，进一步揭示B12对初级生产力和浮游植物生长的影响，并为沿海环境中与营养物质循环有关的概念模型和实际管理决策提供更好的基础。皮？S要求提供资金，以开发使用稳定和时间分辨荧光技术测定沿海海水和沉积物孔隙水中维生素B12的ELISA法。基于免疫亲和固相萃取的流动注射免疫传感器将通过远程控制技术或在船上快速原位测量海水中的超痕量B12。一种新的基于上覆水方法优化的荧光共振能量转移(FRET)平面免疫传感器将被用于原位绘制二维维生素B12在海底沉积物中的分布和运输模式。更广泛的影响：拟议中的工作可能会彻底改变我们分析小生物分子的方式，并可能在化学海洋学以外的领域有重要应用，如化学生态学。该项目有可能开发一种新型传感器，如果成功，将大大有助于我们了解B12在海洋系统中的影响。这些新技术(流动免疫传感器、平面荧光)将被纳入研究生和本科生的课程，以及针对高积极性学生的当地高中推广计划。

项目成果

Planar fluorescence sensors for two-dimensional measurements of H2S distributions and dynamics in sedimentary deposits

• DOI: 10.1016/j.marchem.2013.08.001
• 发表时间: 2013-12-20
• 期刊: MARINE CHEMISTRY
• 影响因子: 3
• 作者: Zhu, Qingzhi;Aller, Robert C.
• 通讯作者: Aller, Robert C.

The weathering of oil after the Deepwater Horizon oil spill: insights from the chemical composition of the oil from the sea surface, salt marshes and sediments

• DOI: 10.1088/1748-9326/7/3/035302
• 发表时间: 2012-07-01
• 期刊: ENVIRONMENTAL RESEARCH LETTERS
• 影响因子: 6.7
• 作者: Liu, Zhanfei;Liu, Jiqing;Wu, Wei
• 通讯作者: Wu, Wei