Analysis of novel plastid proteins targeting nucleic acids: Identification of native RNA/DNA ligands and interacting proteins

靶向核酸的新型质体蛋白分析：天然 RNA/DNA 配体和相互作用蛋白的鉴定

• 批准号: 186136288
• 负责人: Dr. Jeannette Pfalz
• 金额: --
• 依托单位: Lehrstuhl für Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/186136288?language=en
• 关键词: Analysis; novel; plastid; proteins; targeting

Chloroplasts encode a small set of proteins which are however important for the biogenesis of photosynthetic complexes and the gene expression machinery. Their expression requires a variety of nuclear encoded factors. Recently, we have identified two novel proteins involved in plastid gene expression. PTAC12 is a protein of unknown function but has been discovered as component of the transcriptionally active chromosome suggesting a role in transcription or post-transcription. PPR10, a pentatricopeptide repeat protein, acts as RNA binding protein which is involved in RNA stabilization and maturation of photosynthesis genes. Using molecular and biochemical tools the project aims to elucidate the physiological function of PTAC12, to identify its native nucleic acid ligands on a genome-wide scale and to analyze its potential environmental light regulation via redox signals. A proteomic-based approach focuses on the identification of potential protein interaction partners of both PTAC12 and PPR10. New proteins will then be characterized in planta. The project aims to advance understanding of DNA/RNA metabolic processes and to achieve insights into the role of new proteins involved in plastid gene expression.

叶绿体编码一小组蛋白质，但这些蛋白质对于光合复合物的生物发生和基因表达机制很重要。它们的表达需要多种核编码因子。最近，我们发现了两种参与质体基因表达的新蛋白质。 PTAC12 是一种功能未知的蛋白质，但已被发现是转录活性染色体的组成部分，表明其在转录或转录后中发挥作用。 PPR10 是一种五肽重复蛋白，作为 RNA 结合蛋白，参与 RNA 稳定和光合作用基因的成熟。该项目旨在利用分子和生化工具阐明 PTAC12 的生理功能，在全基因组范围内识别其天然核酸配体，并通过氧化还原信号分析其潜在的环境光调节。基于蛋白质组学的方法侧重于鉴定 PTAC12 和 PPR10 的潜在蛋白质相互作用伙伴。然后将在植物中表征新的蛋白质。该项目旨在增进对 DNA/RNA 代谢过程的理解，并深入了解质体基因表达中涉及的新蛋白质的作用。

项目成果

A Major Role for the Plastid-Encoded RNA Polymerase Complex in the Expression of Plastid Transfer RNAs1[W][OPEN]

• DOI: 10.1104/pp.113.228726
• 发表时间: 2013-11
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: R. Williams-Carrier;Reimo Zoschke;Susan Belcher;J. Pfalz;A. Barkan