Phenotypic Heterogeneity of autoinducer-regulated processes in Vibrio harveyi

哈维氏弧菌自诱导剂调节过程的表型异质性

• 批准号: 186440919
• 负责人: Professorin Dr. Kirsten Jung
• 金额: --
• 依托单位: Abteilung Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/186440919?language=en
• 关键词: Phenotypic; Heterogeneity; autoinducer; regulated; processes

Bacteria produce and excrete signaling molecules, so called autoinducers (AIs), which allow them to monitor their population density and/or their environment in a process best known as quorum sensing. The project will explore the molecular basis of phenotypic heterogeneity of AIdependent processes in Vibrio harveyi, one of the best investigated model organism in quorum sensing. This bacterium produces bioluminescence, exoenzymes, siderophores, biofilm, and a type-III secretion system in an AIs-dependent manner. We found phenotypic heterogeneity within an AI-activated wild-type community of V. harveyi with respect to bioluminescence production. Importantly, a heterogeneous population had a higher capacity to produce biofilm. It is the goal of this project to investigate the molecular details of these phenomena by studying: 1. the spatiotemporal appearance of various AI-regulated phenotypes in planktonic cells growing into a biofilm; 2. the molecular components that program the available concentration of the three AIs HAI-1, AI-2 and CAI-1; 3. the molecular switches responsible for (bi-) multi-stability within the complex signal transduction cascade; and 4. the division of work within a bacterial population by using systemic approaches. Solving these questions will improve our understanding of the development of bacterial populations which is relevant for medical and biotechnological applications.

细菌产生和分泌信号分子，即所谓的自诱导物（AI），这使它们能够在一个称为群体感应的过程中监测其种群密度和/或环境。该项目将探索哈维氏弧菌（Vibrio harveyi）中AI依赖过程的表型异质性的分子基础，哈维氏弧菌是群体感应研究中最好的模式生物之一。这种细菌以AIS依赖性方式产生生物发光、胞外酶、铁载体、生物膜和III型分泌系统。我们发现在AI激活的野生型哈维氏弧菌群落中，生物发光产生存在表型异质性。重要的是，异质群体具有更高的产生生物膜的能力。本项目的目标是通过研究这些现象的分子细节：1。生长成生物膜的增殖细胞中各种AI调节表型的时空外观; 2.编程三种AI HAI-1、AI-2和CAI-1的可用浓度的分子组分; 3.负责复杂信号转导级联内的（双）多稳定性的分子开关;和4.通过使用系统方法在细菌群体内进行工作分工。解决这些问题将提高我们对细菌种群发展的理解，这与医学和生物技术应用有关。