CAREER: Next-Generation Flow Cytometry - A New Approach to Cell Heterogeneity

职业:下一代流式细胞术 - 细胞异质性的新方法

基本信息

  • 批准号:
    2422750
  • 负责人:
  • 金额:
    $ 68.1万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2024
  • 资助国家:
    美国
  • 起止时间:
    2024-04-01 至 2025-04-30
  • 项目状态:
    未结题

项目摘要

Metabolism is the biochemical process that supplies energy, molecular building blocks, and chemical signals to control cellular functions essential for life. With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Ku-Lung (Ken) Hsu from the University of Virginia to investigate how individual cells control the metabolism of fats and lipids. Dr. Hsu is developing novel chemical compounds that attach to enzymes (protein catalysts) to probe the metabolism of fats and lipids inside of cells. These probes illuminate how cells are similar or distinct based on their metabolic status. Uncovering distinctive metabolic identities within a massive assortment of cells shows how seemingly identical cells differentiate into specific cell types (e.g. a nerve versus a muscle cell). The metabolic signals discovered through these studies may enable the development of new cell types or properties to push the boundaries of cell engineering. Graduate students receive unique training in chemistry and chemical biology to foster their development as outstanding leaders and role models in society. The research project is integrated with an educational outreach program designed to broadly impact Native American students communities by teaching how lipid (bio)chemistry influences healthy food choices and eating behaviors in society.This research program aims to understand metabolic regulation of cellular function at the single-cell level. The significance of the studies is to establish a fundamental understanding of how identical cells differentiate into functionally distinct cell lineages. The hypothesis is that metabolism imparts asymmetry by providing essential chemical signals that specify a cell's function and fate. Small molecule reporters are integrated with flow cytometry for massively parallel analysis of dynamic metabolic regulation across millions of live cells with unprecedented molecular resolution. Establishing metabolism as a mechanism for asymmetrical specification of cell biology reveals completely new cell phenotypes and has broader impacts in cell biology and engineering. Thus, the research studies are foundational for establishing chemical probes for single-cell analysis that can be combined with modern "omics" methods to open new opportunities for probing cellular function with high resolution.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
新陈代谢是提供能量、分子构件和化学信号以控制生命所必需的细胞功能的生化过程。通过这一奖项,化学系的生命过程化学项目资助弗吉尼亚大学的Ku-loong(Ken)Hsu博士研究单个细胞如何控制脂肪和脂类的代谢。许博士正在开发附着在酶(蛋白质催化剂)上的新型化合物,以探测细胞内脂肪和脂类的新陈代谢。这些探针阐明了细胞如何根据其代谢状态而相似或不同。发现大量细胞中独特的新陈代谢特征,显示了看似相同的细胞如何分化为特定类型的细胞(例如,神经细胞与肌肉细胞)。通过这些研究发现的代谢信号可能会使新细胞类型或特性的开发突破细胞工程的界限。研究生接受独特的化学和化学生物学方面的培训,以培养他们作为社会杰出领导者和榜样的发展。该研究项目与一个教育推广项目相结合,旨在通过教授脂质(生物)化学如何影响社会中健康的食物选择和饮食行为来广泛影响美国原住民学生社区。该研究项目旨在了解单细胞水平上细胞功能的新陈代谢调节。这些研究的意义是建立一个基本的理解,即相同的细胞如何分化成功能不同的细胞系。假说是,新陈代谢通过提供指定细胞功能和命运的基本化学信号而产生不对称性。小分子报告器与流式细胞仪相结合,以前所未有的分子分辨率对数百万活细胞的动态代谢调节进行大规模并行分析。将代谢建立为细胞生物学不对称规范的一种机制,揭示了全新的细胞表型,并在细胞生物学和工程学中具有更广泛的影响。因此,这些研究是建立用于单细胞分析的化学探针的基础,这些探针可以与现代“组学”方法相结合,为以高分辨率探测细胞功能开辟新的机会。这一奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Ku-Lung Hsu其他文献

Emerging opportunities for intact and native protein analysis using chemical proteomics
利用化学蛋白质组学进行完整和天然蛋白质分析的新兴机遇
  • DOI:
    10.1016/j.aca.2024.343551
  • 发表时间:
    2025-02-08
  • 期刊:
  • 影响因子:
    6.000
  • 作者:
    Alexis N. Edwards;Ku-Lung Hsu
  • 通讯作者:
    Ku-Lung Hsu
Acetaminophen inhibits diacylglycerol lipase synthesis of 2-arachidonoyl glycerol: Implications for nociception
对乙酰氨基酚抑制 2-花生四烯酰甘油的二酰基甘油脂肪酶合成:对伤害感受的影响
  • DOI:
    10.1016/j.xcrm.2025.102139
  • 发表时间:
    2025-06-17
  • 期刊:
  • 影响因子:
    10.600
  • 作者:
    Michaela Dvorakova;Taryn Bosquez-Berger;Jenna Billingsley;Natalia Murataeva;Taylor Woodward;Emma Leishman;Anaëlle Zimmowitch;Anne Gibson;Jim Wager-Miller;Ruyi Cai;Shangxuan Cai;Tim Ware;Ku-Lung Hsu;Yulong Li;Heather Bradshaw;Ken Mackie;Alex Straiker
  • 通讯作者:
    Alex Straiker
DAGL-Beta Functions as a PUFA-Specific Triacylglycerol Lipase in Macrophages
  • DOI:
    10.1016/j.chembiol.2020.01.005
  • 发表时间:
    2020-03-19
  • 期刊:
  • 影响因子:
  • 作者:
    Myungsun Shin;Timothy B. Ware;Ku-Lung Hsu
  • 通讯作者:
    Ku-Lung Hsu

Ku-Lung Hsu的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Ku-Lung Hsu', 18)}}的其他基金

CAREER: Next-Generation Flow Cytometry - A New Approach to Cell Heterogeneity
职业:下一代流式细胞术 - 细胞异质性的新方法
  • 批准号:
    1942467
  • 财政年份:
    2020
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Standard Grant

相似国自然基金

Next Generation Majorana Nanowire Hybrids
  • 批准号:
  • 批准年份:
    2020
  • 资助金额:
    20 万元
  • 项目类别:

相似海外基金

CAREER: Next-generation Logic, Memory, and Agile Microwave Devices Enabled by Spin Phenomena in Emergent Quantum Materials
职业:由新兴量子材料中的自旋现象实现的下一代逻辑、存储器和敏捷微波器件
  • 批准号:
    2339723
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
CAREER: Securing Next-Generation Transportation Infrastructure: A Traffic Engineering Perspective
职业:保护下一代交通基础设施:交通工程视角
  • 批准号:
    2339753
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Standard Grant
CAREER: Next-Generation Methods for Statistical Integration of High-Dimensional Disparate Data Sources
职业:高维不同数据源统计集成的下一代方法
  • 批准号:
    2422478
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
CAREER: LoRa Enabled Space-air-ground Integrated Networks for Next-Generation Agricultural IoT
职业生涯:LoRa 支持下一代农业物联网的天地一体化网络
  • 批准号:
    2338976
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
CAREER: Next-generation protease inhibitor discovery with chemically diversified antibodies
职业:利用化学多样化的抗体发现下一代蛋白酶抑制剂
  • 批准号:
    2339201
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
CAREER: Next Generation Online Resource Allocation
职业:下一代在线资源分配
  • 批准号:
    2340306
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Standard Grant
CAREER: Non-Local Metamaterials and Metasurfaces for Next Generation Non-Reciprocal Acoustic Devices
职业:下一代非互易声学器件的非局域超材料和超表面
  • 批准号:
    2340782
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Standard Grant
CAREER: Next Generation of High-Level Synthesis for Agile Architectural Design (ArchHLS)
职业:下一代敏捷架构设计高级综合 (ArchHLS)
  • 批准号:
    2338365
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
CAREER: Engineering next-generation adrenal gland organoids
职业:设计下一代肾上腺类器官
  • 批准号:
    2335133
  • 财政年份:
    2024
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
CAREER: Next-generation Rhizosphere Monitoring - Non-invasive Plant Phenotyping and Health Monitoring Using the Light-piping Properties of Plant Stems
职业:下一代根际监测 - 利用植物茎的光管特性进行非侵入性植物表型和健康监测
  • 批准号:
    2238365
  • 财政年份:
    2023
  • 资助金额:
    $ 68.1万
  • 项目类别:
    Continuing Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了