Structure of mitochondrial complex I and conformational coupling between electron transfer and proton translocation

线粒体复合物I的结构及电子转移与质子易位之间的构象耦合

• 批准号: 186638795
• 负责人: Professor Dr. Volker Zickermann
• 金额: --
• 依托单位: Arbeitsgruppe Strukturelle Bioenergetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/186638795?language=en
• 关键词: Structure; mitochondrial; complex; conformational; coupling

Proton pumping NADH:ubiquinone oxidoreductase (mitochondrial complex I) is a very large membrane protein with a key function in energy metabolism. We have accomplished the first X-ray crystallographic analysis of the complete eukaryotic enzyme complex comprising 40 subunits with a mass of almost 1 MDa. Structural information at higher resolution and extended functional characterization will be needed in the future to fully understand its mechanism. We plan to crystallize and determine the structure of stable and functional subcomplexes recently obtained in our laboratory and to use a structure guided fluorescence energy transfer (FRET) approach to investigate functionally critical structural changes during turnover of complex I.

质子泵NADH：泛醌氧化还原酶（线粒体复合物I）是一种非常大的膜蛋白，在能量代谢中具有关键功能。我们已经完成了完整的真核生物酶复合物的第一个X-射线晶体学分析，包括40个亚基，质量几乎为1 MDa。未来将需要更高分辨率的结构信息和扩展的功能表征，以充分了解其机制。我们计划结晶和确定最近在我们的实验室获得的稳定和功能的亚复合物的结构，并使用结构引导的荧光能量转移（FRET）的方法来研究功能关键的结构变化，在营业额的复合物I。

项目成果

Mechanistic insight from the crystal structure of mitochondrial complex I

• DOI: 10.1126/science.1259859
• 发表时间: 2015-01-02
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Zickermann, Volker;Wirth, Christophe;Brandt, Ulrich
• 通讯作者: Brandt, Ulrich

The LYR protein subunit NB4M/NDUFA6 of mitochondrial complex I anchors an acyl carrier protein and is essential for catalytic activity

• DOI: 10.1073/pnas.1322438111
• 发表时间: 2014-04-08
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Angerer, Heike;Radermacher, Michael;Zickermann, Volker
• 通讯作者: Zickermann, Volker