Apical secrection and assembly of extracellular matrix during Drosophila oogenesis

果蝇卵子发生过程中细胞外基质的顶端分泌和组装

• 批准号: 18714114
• 负责人: Professor Dr. Christian Dahmann
• 金额: --
• 依托单位: Professur für Systembiologie und Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/18714114?language=en
• 关键词: Apical; secrection; assembly; extracellular; matrix

The polarized secretion and assembly of extracellular matrices are fundamental processes important for cellular and organismal function. However, little is known how these matrices are assembled in vivo. The Drosophila eggshell is a specialized extracellular matrix secreted by polarized epithelial follicle cells and has served as an excellent model system to analyze extracellular matrix assembly in vivo. We have recently identified mutations in the Drosophila gene cad99C and provide several lines of evidence that these mutants are defective in forming a proper eggshell. Cad99C is the Drosophila ortholog of human Protocadherin 15, a protein of the family of Ca2+- dependent cell adhesion molecules that is etiologically associated with deafness (Usher syndrome type I). The aim of the proposed project is to determine by a combination of molecular and biochemical approaches as well as ultra-structural analysis how Cad99C contributes to the formation of the Drosophila eggshell. It is expected that our analysis will provide novel insights into mechanisms used by polarized cells to apically secrete and assemble extracellular matrices and, in addition, into the molecular and cell biological basis of Usher syndrome type I.

细胞外基质的极化分泌和组装是对细胞和有机体功能重要的基本过程。然而，人们对这些基质在体内如何组装知之甚少。果蝇蛋壳是一种由极化上皮滤泡细胞分泌的特殊细胞外基质，已成为分析体内细胞外基质组装的优秀模型系统。我们最近发现了果蝇基因 cad99C 中的突变，并提供了多种证据表明这些突变体在形成适当的蛋壳方面存在缺陷。 Cad99C 是人原钙粘蛋白 15 的果蝇直系同源物，原钙粘蛋白 15 是一种 Ca2+ 依赖性细胞粘附分子家族的蛋白质，在病因学上与耳聋（I 型亚瑟综合征）相关。该项目的目的是通过结合分子和生化方法以及超微结构分析来确定 Cad99C 如何促进果蝇蛋壳的形成。预计我们的分析将为极化细胞顶端分泌和组装细胞外基质的机制提供新的见解，此外，还将为 I 型亚瑟综合征的分子和细胞生物学基础提供新的见解。