Dynamics of cell contacts during cell sorting at compartment boundaries in Drosophila

果蝇隔室边界细胞分选过程中细胞接触的动态

• 批准号: 200510564
• 负责人: Professor Dr. Christian Dahmann
• 金额: --
• 依托单位: Professur für Systembiologie und Genetik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200510564?language=en
• 关键词: Dynamics; cell; contacts; during; sorting

The partitioning of proliferating tissues into groups of non-intermingling cells, termed compartments, is crucial for animal development. Sharp and straight boundaries between compartments are important for the positioning of signalling centres and thus for growth and patterning. Cell rearrangements caused by cell division challenge, however, straight and sharp compartment boundaries. Our recent findings indicate that differential Myosin II-dependent mechanical tension on cell junctions is important for maintaining straight interfaces between compartments. The aim of this project is to understand how such a differential mechanical tension guides the rearrangement of cells after cell division to maintain straight compartment boundaries. We will combine live imaging, Fluorescence recovery after photobleaching (FRAP), and laser nanosurgery with quantitative image analysis to determine and to correlate the dynamics of cell junctions with the dynamics of the Myosin II motor protein and the dynamics of mechanical tension in cells dividing along the anteroposterior compartment boundary of the developing Drosophila wing. The functional significance of junctional dynamics along the anteroposterior compartment boundary will be tested in experiments using mutants for Myosin II. We expect that this project will provide novel mechanistic insights into the cellular mechanisms that guide cell sorting during animal development.

增殖组织分裂成非混合细胞群，称为隔室，对动物发育至关重要。隔室之间尖锐和笔直的边界对于信号中心的定位以及生长和模式是重要的。细胞分裂引起的细胞重排挑战，然而，笔直和尖锐的室边界。我们最近的研究结果表明，细胞连接上依赖于肌球蛋白ii的不同机械张力对于维持室之间的直界面是重要的。这个项目的目的是了解这种不同的机械张力如何引导细胞分裂后的细胞重排，以保持细胞间的直线边界。我们将结合实时成像、光漂白后荧光恢复（FRAP）和激光纳米手术与定量图像分析，以确定细胞连接的动力学与肌球蛋白II运动蛋白的动力学以及细胞沿发育中的果蝇翅膀前后隔室边界分裂的机械张力动力学，并将其联系起来。沿着前后室边界的连接动力学的功能意义将在使用肌球蛋白II突变体的实验中进行测试。我们期望这个项目将为指导动物发育过程中细胞分选的细胞机制提供新的机制见解。