Collaborative Research: MIP: Pressure Influences on Microbial Life in the Puerto Rico Trench
合作研究:MIP:压力对波多黎各海沟微生物生命的影响
基本信息
- 批准号:0801793
- 负责人:
- 金额:$ 39.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-08-15 至 2012-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Pressure is a physical parameter which influences the evolution and distribution of microbial life. Far from Earth?s surface, deep ocean trenches represent the upper boundaries of pressure in the biosphere. This is where scientists can study high pressure adaptations in their most extreme forms. No studies have yet characterized the diversity of microbial life in trenches. In order to do this, Doug Bartlett from UC San Diego and Wilford Schmidt from Univ. Puerto Rico-Mayaguez, will be using an untethered, free-falling, pressured-hardened device to sample bacterial and archaeal life in the deepest trench in the Atlantic Ocean, the Puerto Rico Trench (PRT), at a depth of 8.4 km (5.2 mi). They will profile the ambient environment in which these prokaryotes live (pressure, temperature, salinity, and current velocity). They will also chemically analyze the trench seawater and carry out total microbial cell counts and probe-based counts of particular microbial groups, perform DNA extractions and isolate and culture deep-dwelling microorganisms. It is these last two goals that are at the heart of the project. They will use the DNA to characterize the trench microbial community via indicator genes and community fingerprint techniques. Cultivation of these high pressuring loving microbes (called ?piezophiles?) is difficult since the culture vessels are under very high pressure and the samples are difficult to manipulate. The Bartlett laboratory has the expertise to culture piezophiles and so a major effort will be made to isolate pure cultures of some of the major microbial groups observed in the PRT, including members of the class ?Alphaproteobacteria?, order Flavobacteriales, order Planctomycetales, and subclass marine group I Crenarchaeota. They will also characterize the physiology and taxonomy of particular isolates. In the long term, these investigators are interested in studying the effect of decompression or pressurization on deep-trench or surface-water microbial assemblages because they are interested in understanding the unique abilities of piezophiles to survive and grow under very high pressures. Bartlett and Schmidt will develop outreach programs for pre-college and university students, in Puerto Rico and in southern California, to teach them about this research. Their efforts will include the development of a marine microbiology laboratory class. They will also create highlights from their research for the Birch Aquarium's Deep Ocean Exhibit.
压力是影响微生物生命演化和分布的物理参数。 远离地球?在地球表面,深海海沟代表了生物圈压力的上限。科学家们可以在这里研究最极端形式的高压适应。 目前还没有研究表明战壕中微生物的多样性。 为了做到这一点,来自加州大学圣地亚哥分校的Doug Bartlett和来自波多黎各-马亚圭斯大学的Wilford施密特将使用一种无绳、自由下落、加压硬化的设备,在大西洋最深的海沟,即波多黎各海沟(PRT),8.4公里(5.2英里)深处采集细菌和古生物样本。 他们将描绘这些原核生物生活的周围环境(压力,温度,盐度和流速)。 他们还将对海沟海水进行化学分析,并对特定微生物群进行总微生物细胞计数和基于探针的计数,进行DNA提取,并分离和培养深居微生物。 最后两个目标是该项目的核心。他们将使用DNA通过指示基因和群落指纹技术来表征海沟微生物群落。 培养这些高压力的爱好微生物(称为?piezophiles?)这是困难的,因为培养容器处于非常高的压力下并且样品难以操作。 巴特利特实验室具有培养嗜压菌的专业知识,因此将做出重大努力,分离PRT中观察到的一些主要微生物组的纯培养物,包括类?α变形菌?,黄杆菌目、浮菌目和海生泉古菌亚纲I组。 他们还将描述特定分离株的生理学和分类学特征。 从长远来看,这些研究人员有兴趣研究减压或加压对深沟或地表水微生物群落的影响,因为他们有兴趣了解嗜压生物在非常高的压力下生存和生长的独特能力。 巴特利特和施密特将为波多黎各和加州南部的大学预科生和大学生开发外展项目,向他们传授这项研究。他们的努力将包括发展一个海洋微生物学实验班。他们还将从他们的研究中为桦树水族馆的深海展览创造亮点。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Douglas Bartlett其他文献
Low temperature and high hydrostatic pressure have compounding effects on marine microbial motility
- DOI:
10.1016/j.bpj.2022.11.2829 - 发表时间:
2023-02-10 - 期刊:
- 影响因子:
- 作者:
Kelli K. Mullane;Masayoshi Nishiyama;Tatsuo Kurihara;Douglas Bartlett - 通讯作者:
Douglas Bartlett
Transcriptomic analysis reveals common adaptation mechanisms under different stresses for moderately piezophilic bacteria
转录组分析揭示了中度嗜压细菌在不同压力下的常见适应机制
- DOI:
10.1007/s00248-020-01609-3 - 发表时间:
2020 - 期刊:
- 影响因子:3.6
- 作者:
Han Wang;Yu Zhang;Douglas Bartlett;Xiang Xiao - 通讯作者:
Xiang Xiao
A nearly uniform distributional pattern of heterotrophic bacteria in the Mariana
- DOI:
https://doi.org/10.1016/j.dsr.2018.10.002 - 发表时间:
2018 - 期刊:
- 影响因子:
- 作者:
Jiwei Tian;Lu Fan;Haodong Liu;Jiwen Liu;Yi Li;Qilong Qin;Zheng Gong;Hongtao Chen;Zhongbin Sun;Li Zou;Xuchen Wang;Hongzhou Xu;Douglas Bartlett;Min Wang;Min Wang;Xiao-Hua Zhang;Chuanlun Lun Zhang - 通讯作者:
Chuanlun Lun Zhang
Douglas Bartlett的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Douglas Bartlett', 18)}}的其他基金
Collaborative Research: Influence of pressure on microbial communities in subseafloor sediment at hadal, abyssal, bathyal, and shelf water depths
合作研究:压力对深渊、深海、深海和陆架水深海底沉积物中微生物群落的影响
- 批准号:
2048927 - 财政年份:2021
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
Collaborative Research: Transcriptional Adaptation and Response to Pressure
合作研究:转录适应和对压力的反应
- 批准号:
2019455 - 财政年份:2020
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
Patterns of Microbial Community Structure Within and Between Hadal Environments
深渊环境内部和之间的微生物群落结构模式
- 批准号:
1536776 - 财政年份:2015
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
US-EC Workshop on Marine Genomics: Next Generation Scientists for Next Generation Sequencing, October 10-12, 2010, Washington, DC
US-EC 海洋基因组学研讨会:下一代科学家进行下一代测序,2010 年 10 月 10-12 日,华盛顿特区
- 批准号:
1068643 - 财政年份:2010
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
2008 EC-US Workshop on Marine Genomics in Association with CIESM: at the Interface of Marine Microbial Ecology and Biotechnological Applications
2008 年欧盟-美国海洋基因组学研讨会与 CIESM 联合举办:海洋微生物生态学与生物技术应用的交汇处
- 批准号:
0841547 - 财政年份:2008
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
SGER: Life at Depth: A Comparative Genomics Perspective
SGER:深度生命:比较基因组学视角
- 批准号:
0544524 - 财政年份:2006
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
Functional Genomics of High Pressure Adaptation
高压适应的功能基因组学
- 批准号:
0237059 - 财政年份:2003
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
Developing and Testing of Bioreactors for Methane Hydrate Biogeochemical Studies in the Laboratory
实验室甲烷水合物生物地球化学研究生物反应器的开发和测试
- 批准号:
0096475 - 财政年份:2001
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
ToxR/S and Unsaturated Fatty Acid Regulation in Photobacterium profundum Strain SS9
深光杆菌菌株 SS9 中的 ToxR/S 和不饱和脂肪酸调节
- 批准号:
9974528 - 财政年份:1999
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
相似国自然基金
Research on Quantum Field Theory without a Lagrangian Description
- 批准号:24ZR1403900
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
Cell Research
- 批准号:31224802
- 批准年份:2012
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research
- 批准号:31024804
- 批准年份:2010
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research (细胞研究)
- 批准号:30824808
- 批准年份:2008
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
- 批准号:10774081
- 批准年份:2007
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
Collaborative Research: MIP: How Responsive are Bacterial Endosymbionts to Physiological and Eological Variation in Their Ant Hosts?
合作研究:MIP:细菌内共生体对其蚂蚁宿主的生理和生态变化的反应如何?
- 批准号:
1103113 - 财政年份:2010
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
Collaborative Research: MIP: Pressure Influences on Microbial Life in the Puerto Rico Trench
合作研究:MIP:压力对波多黎各海沟微生物生命的影响
- 批准号:
0801809 - 财政年份:2008
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
COLLABORATIVE RESEARCH (MIP): Microbial Interactions at Cold Seeps: Characterizing C2-C4 anaerobic hydrocarbon degradation and its influence on AMO and sulfate reduction
合作研究 (MIP):冷泉微生物相互作用:表征 C2-C4 厌氧碳氢化合物降解及其对 AMO 和硫酸盐还原的影响
- 批准号:
0702504 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
Collaborative Research (MIP): Microbial Interactions at Cold Seeps: Characterizing C2-C4 anaerobic hydrocarbon degradation and its influence on AMO and sulfate reduction.
合作研究 (MIP):冷泉微生物相互作用:表征 C2-C4 厌氧碳氢化合物降解及其对 AMO 和硫酸盐还原的影响。
- 批准号:
0702080 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
COLLABORATIVE RESEARCH: MIP: The Thermo-acidophilic Cyanidiales: Genetic and Physiological Diversity in Relation to Environment and Seasonal Change in Yellowstone National Park
合作研究:MIP:嗜热酸性氰化物目:黄石国家公园与环境和季节变化相关的遗传和生理多样性
- 批准号:
0702135 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant
COLLABORATIVE RESEARCH: MIP: The Termo-acidophilic Cyanidiales: Genetic and Physiological Diversity in Relation to Environment and Seasonal Change in Yellowstone National Park
合作研究:MIP:嗜酸氰虫目:黄石国家公园与环境和季节变化相关的遗传和生理多样性
- 批准号:
0702177 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
Collaborative Research: MIP: How Responsive are Bacterial Endosymbionts to Physiological and Eological Variation in Their Ant Hosts?
合作研究:MIP:细菌内共生体对其蚂蚁宿主的生理和生态变化的反应如何?
- 批准号:
0604177 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
COLLABORATIVE RESEARCH: MIP: The Thermo-acidophilic Cyanidiales: Genetic and Physiological Diversity in Relation to Environment and Seasonal Change in Yellowstone National Park
合作研究:MIP:嗜热酸性氰化物目:黄石国家公园与环境和季节变化相关的遗传和生理多样性
- 批准号:
0702212 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
Collaborative Research: MIP: Bacterial Interactions and Processes that Regulate Bacterial Biodiversity on Marine Organic Particles
合作研究:MIP:调节海洋有机颗粒上细菌生物多样性的细菌相互作用和过程
- 批准号:
0729594 - 财政年份:2007
- 资助金额:
$ 39.22万 - 项目类别:
Continuing Grant
RUI: Collaborative Research: MIP : Lake Huron Sinkholes - Microbial Composition and Processes in Biogeochemical Hotspots
RUI:合作研究:MIP:休伦湖污水坑 - 生物地球化学热点地区的微生物组成和过程
- 批准号:
0603944 - 财政年份:2006
- 资助金额:
$ 39.22万 - 项目类别:
Standard Grant