Integrated Nanosystem Combining Engineered Nanopore Devices and Hierarchical Model-Based Diagnosis Algorithms for Ultra-Rapid Biomolecule Analysis

集成纳米系统结合工程纳米孔器件和基于分层模型的诊断算法，用于超快速生物分子分析

• 批准号: 0801829
• 负责人: Sankar Nair
• 金额: $ 45.03万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-15 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0801829&HistoricalAwards=false
• 关键词: Integrated; Nanosystem; Combining; Engineered; Nanopore

Objective:The objective of this research is to create a nanoscale system that achieves ultra-rapid, high-resolution analysis of biomolecules such as nucleic acids and proteins. The approach is based on a combination of nanopore device fabrication, predictive physics-based diagnostic algorithms to control biomolecule transport in the device, and integration of these subsystems to demonstrate high-speed biomolecule analysis with much higher resolution than currently possible. Intellectual Merit:This work addresses the challenge of developing nucleic acid and protein sequencing platforms that achieve orders-of-magnitude savings in time and cost. This is essential to transform current understanding of the genetic basis of disease and thereby revolutionize many areas of medicine and biotechnology. This work takes an integrated approach to the critical issues of device fabrication and high-performance operation. It combines a wafer-scale process (based on advanced electron-beam and atomic layer deposition techniques) to fabricate the first reproducible arrays of engineered nanopore devices, with a novel computational diagnosis platform that couples hierarchically detailed simulation engines and mathematical algorithms to adaptively refine the nanopore device performance for ?single-nucleotide? resolution. Broader Impact:This program will develop interdisciplinary and diverse science and technology manpower by training three graduate and nine undergraduate students across five engineering and science disciplines, enhanced by collaborations with national laboratories. Three of the undergraduate trainees will be recruited from underrepresented minorities by active collaboration with a campus program for creating research opportunities for underrepresented minorities. The project will develop hands-on modules for undergraduate, graduate, and continuing education courses in circuit diagnosis, nanofabrication, and nanosystems.

目的：本研究的目标是建立一个纳米系统，实现超快速，高分辨率的生物分子，如核酸和蛋白质的分析。该方法基于纳米孔设备制造，基于预测物理学的诊断算法的组合，以控制设备中的生物分子运输，以及这些子系统的集成，以证明具有比目前可能的高得多的分辨率的高速生物分子分析。智力优势：这项工作解决了开发核酸和蛋白质测序平台的挑战，实现了时间和成本的数量级节省。这对于改变目前对疾病遗传基础的理解，从而彻底改变医学和生物技术的许多领域至关重要。这项工作需要一个综合的方法来解决器件制造和高性能操作的关键问题。它结合了晶圆级的过程（基于先进的电子束和原子层沉积技术），以制造工程纳米孔设备的第一个可重复的阵列，与一种新的计算诊断平台，耦合层次详细的模拟引擎和数学算法，自适应地完善纳米孔设备的性能？单核苷酸分辨率更广泛的影响：该计划将通过培训五个工程和科学学科的三名研究生和九名本科生来开发跨学科和多样化的科学技术人才，并通过与国家实验室的合作来加强。其中三名本科生将通过与校园项目积极合作，从代表性不足的少数族裔中招募，为代表性不足的少数族裔创造研究机会。该项目将为电路诊断、纳米制造和纳米系统的本科生、研究生和继续教育课程开发实践模块。