Mechanism and Dynamics of Protein Interactions with Polymer Brushes

蛋白质与聚合物刷相互作用的机制和动力学

• 批准号: 0804113
• 负责人: Deborah Leckband
• 金额: $ 33万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-10-01 至 2012-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0804113&HistoricalAwards=false
• 关键词: Mechanism; Dynamics; Protein; Interactions; Polymer

ID: MPS/DMR/BMAT(7623) 0804113 PI: Leckband, Deborah ORG: Illinois-Urbana/ChampaignTitle: Mechanism and Dynamics of Protein Interactions with Polymer BrushesINTELLECTUAL MERIT: The proposal aims to identify engineering design rules for controlling protein and cell interactions with biomaterials. A central and persistent problem confronting biomaterials design is the uncontrolled adsorption of proteins to material surfaces. There is currently no general consensus on how to design a protein-resistant coating. A major gap in current knowledge is the limited understanding of the basic mechanisms of protein adsorption to polymer interfaces in aqueous media. Absent this information, it is difficult to define engineering approaches to control specific behavior. This research plan will address this knowledge gap by using a combination of synthetic chemistry and complementary experimental approaches that probe the mechanisms by which proteins interact with grafted polymers. In Objective 1, polymer gradients will be used as high throughput screening platforms to identify polymer brush properties that support protein adsorption. In Objective 2, a combination of fluorescence techniques will quantify the diffusivity of adsorbed proteins as a function of the brush parameters. In Objective 3, neutron reflectivity measurements with deuterated proteins will determine where adsorbed proteins localize relative to the polymer brush. Finally, Objective 4 will quantify the magnitude and range of attractive and repulsive forces between proteins and polymers that are responsible for protein adsorption behavior. The work will help to develop an understanding of the interactions of proteins with polymer brushes and could advance the design and preparation of surfaces resistant to protein adsorption and biofouling.BROADER IMPACTS: Success of this project will shed new and useful light on the mechanisms by which polymer brush coatings may protect surfaces from unwanted protein adsorption. With respect to broadening participation, the project will continue the PI's on-going efforts to involve students from underrepresented groups in science and engineering in the research endeavor. These efforts will include inclusion of females and underrepresented minorities in graduate level research and participation in the Summer Research Opportunity Program that provides opportunities for minority undergraduate students to participate in research.

ID：MPS/DMR/BMAT（7623）0804113 PI：Leckband，Deborah ORG：Illinois-Urbana/ChampaignTitle：蛋白质与聚合物刷相互作用的机制和动力学智力优势：该提案旨在确定控制蛋白质和细胞与生物材料相互作用的工程设计规则。生物材料设计面临的一个中心和持久的问题是蛋白质在材料表面的不受控制的吸附。目前对于如何设计抗蛋白质涂层没有普遍共识。 目前知识的一个主要差距是对蛋白质吸附到水性介质中聚合物界面的基本机制的理解有限。如果没有这些信息，就很难定义控制特定行为的工程方法。该研究计划将通过使用合成化学和互补实验方法的组合来解决这一知识差距，这些方法可以探测蛋白质与接枝聚合物相互作用的机制。在目标1中，聚合物梯度将被用作高通量筛选平台，以确定支持蛋白质吸附的聚合物刷特性。在目标2中，荧光技术的组合将量化作为刷子参数的函数的吸附蛋白质的扩散率。在目标3中，中子反射率测量与氘代蛋白质将确定吸附蛋白质定位相对于聚合物刷。最后，目标4将量化蛋白质和聚合物之间的吸引力和排斥力的大小和范围，这些吸引力和排斥力是蛋白质吸附行为的原因。 这项工作将有助于发展的蛋白质与聚合物刷的相互作用的理解，并可以推进设计和制备的表面抗蛋白质吸附和biofouling.BroADER重要：这个项目的成功将揭示新的和有用的光的机制，聚合物刷涂层可以保护表面免受不必要的蛋白质吸附。 在扩大参与方面，该项目将继续PI的持续努力，使科学和工程领域代表性不足的学生参与研究奋进。 这些努力将包括将女性和代表性不足的少数民族纳入研究生水平的研究，并参与夏季研究机会计划，为少数民族本科生提供参与研究的机会。