Multifunctional Mesoporous Silica Nanoparticles for Intracellular Controlled Release

用于细胞内控释的多功能介孔二氧化硅纳米粒子

• 批准号: 0809521
• 负责人: Brian Trewyn
• 金额: $ 39万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0809521&HistoricalAwards=false
• 关键词: Multifunctional; Mesoporous; Silica; Nanoparticles; Intracellular

This award in the Inorganic, Bioinorganic and Organometallic Chemistry program in the Division of Chemistry and the Biomaterials program in the Division of Materials research supports Professor Victor Lin at Iowa State University to synthesize biocompatible, multi-functionalized mesoporous silica nanoparticle (MSN) materials with well-defined particle morphology and tunable surface properties for efficient penetration across different cell membranes. A series of pore-capping and uncapping strategies will be developed that can be triggered by cellular chemicals and enzymes for studying intracellular controlled release of a variety of guest molecules, such as drugs, imaging agents, peptides, proteins, and nucleotides, inside of animal and plant cells. The specific objectives are to: 1) Synthesize biocompatible mesoporous silica nanoparticles for controlling membrane trafficking, 2) Fine-tune surface properties of MSNs for loading and release of chemicals, 3) Develop an arsenal of different pore-capping and uncapping strategies, 4) Study mechanisms of endocytosis, intracellular transportation, and controlled release. Various interactions between the multifunctional MSNs with different particle morphologies and animal and plant cells will be studied in vitro. These interactions include: the adsorption of MSNs to cell membranes, the mechanism and kinetics of internalization, the intracellular localization of internalized MSN, the biocompatibility, and the effects of MSNs on cellular metabolism. A series of imaging, cytochemical, and cell sorting techniques will be employed to study the dynamics of cellular uptake of the MSNs and the kinetics of intracellular controlled release of biologically active molecules from MSNs. This research offers new nanomaterials for chemists, biologists, and material scientists to study the chemical nature and biochemistry inside living cells. The interdisciplinary work will also provide valuable training to the students working on this project. Other broader impacts include: The development of new curricular materials for graduate and undergraduate courses and outreach to local elementary and high schools.

化学、生物无机和金属有机化学项目和材料研究部门生物材料项目的这一奖项支持爱荷华州立大学的Victor Lin教授合成具有良好颗粒形态和可调表面特性的生物兼容、多功能介孔二氧化硅纳米颗粒(MSN)材料，以有效地穿透不同的细胞膜。将开发一系列毛孔封顶和去封顶策略，这些策略可以由细胞化学物质和酶触发，用于研究各种客体分子在动植物细胞内的受控释放，如药物、成像剂、多肽、蛋白质和核苷酸。其具体目标是：1)合成生物相容的介孔二氧化硅纳米颗粒，用于控制膜的转运；2)微调MSN的表面性质，用于装载和释放化学物质；3)开发不同的孔帽和解帽策略的武器库；4)研究内吞作用、细胞内运输和控制释放的机制。具有不同颗粒形态的多功能MSN与动植物细胞之间的各种相互作用将在体外进行研究。这些相互作用包括：MSN在细胞膜上的吸附，内化的机理和动力学，内化的MSN在细胞内的定位，生物相容性，以及MSN对细胞代谢的影响。一系列的成像、细胞化学和细胞分选技术将被用来研究细胞摄取MSN的动力学和从MSN中控制释放生物活性分子的动力学。这项研究为化学家、生物学家和材料科学家研究活细胞内的化学性质和生物化学提供了新的纳米材料。跨学科的工作也将为参与这个项目的学生提供有价值的培训。其他更广泛的影响包括：为研究生和本科课程编写新的课程材料，并推广到当地的小学和高中。