An Integrated View of Polarity Establishment in Fucoid Zygotes

Darryl Louis Kropf提案#0817045标题：岩藻类合子极性建立的综合观点控制褐藻发育的机制的研究一直受到缺乏分子和细胞工具的阻碍。 特别是，已经证明开发允许研究人员将感兴趣的分子引入褐藻细胞的技术非常困难。该项目将开发一种用于褐藻的分子传递系统。该技术使用充满感兴趣分子的脂质囊泡。脂质囊泡与藻类细胞融合，并将其内容物输送到细胞中。初步结果表明，特定的分子可以通过这种方式传递，下一步是证明它对许多不同类型的分子起作用。 这项技术将是真正的变革性的，因为它将使科学家首次研究单个分子的功能。预计这种技术将适用于许多不同的藻类，也许也适用于其他类型的细胞。一名博士生和四名本科生将利用这笔资金接受培训。

Darryl Louis KropfProposal # 0817045Title: An Integrated View of Polarity Establishment in Fucoid ZygotesResearch into the mechanisms that control brown algal development has been hampered by a lack of molecular and cellular tools. In particular, it has proven to be very difficult to develop techniques that allow researchers to introduce molecules of interest into brown algal cells. This project will develop a molecular delivery system for use with brown algae. The technique uses lipid vesicles filled with the molecule of interest. The lipid vesicles fuse with the algal cell and deliver their contents into the cell. Initial results show that specific molecules can be delivered in this way, and the next step is to show that it works with many different kinds of molecules. This technique will be truly transformative in that it will allow scientists to study the function of individual molecules for the first time. It is anticipated that this technique will be useful with many different algae, and perhaps with other types of cells as well. A PhD student and four undergraduates will be trained with this funding.