Polarization of Fucus Zygotes and Protoplasts

墨角藻合子和原生质体的极化

• 批准号: 8904770
• 负责人: Darryl Kropf
• 金额: $ 23.72万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904770&HistoricalAwards=false
• 关键词: Polarization; Fucus; Zygotes; Protoplasts

Investigation of the earliest events in embryonic polarization in plants have focused on two genera of closely related brown algae, Fucus and Pelvetia. Current working hypotheses postulate that during polarization ion channels in the plasma membrane migrate to one end of the egg. This asymmetry gives rise to net influx in the region of channel accumulation and net efflux from the other end. Thus, an ionic current flows through the egg and denotes formation of the developmental axis. As a consequence of the current, cytoplasmic voltage and ionic gradients are established and these are thought to regulate regional differentiation within the egg. One major aspect of this attractive model that has yet to be scrutinized is whether a physical reorganization of plasma membrane components (e.g.ion channels) takes place as the axis is established. Dr. Kropf has recently shown that Fucus protoplasts made from young zygotes can be polarized with unidirectional light. This provides direct access to the plasma membrane during axis establishment. He will examine rearrangements of membrane receptors on the surface of protoplasts undergoing polarization and investigate ionic currents generated by the membrane asymmetries in Fucus zygotes and in photopolarized protoplasts. The ultimate goal is to monitor the spatial distributions of ionic currents, plasma membrane receptors and F-actin in single cells as they establish an axis. %%% The establishment of the axis (i.e. which end is which) of an organism early in development is a fundamental first step in the pattern formation of that organism. This event has been hard to analyze in plants. Dr. Kropf is studying this process in the brown algae because of the experimental advantages it presents relative to many other plants.

胚胎极化最早期事件的研究 植物集中在两个密切相关的褐藻属， 墨角藻和Pelvetia。目前的工作假设假定， 在极化过程中，质膜中的离子通道迁移 鸡蛋的一端。这种不对称性导致净流入， 通道积累区和另一个通道净流出区 端因此，离子电流流过鸡蛋， 发展轴的形成。原因是 建立电流、细胞质电压和离子梯度 这些被认为是调节区域分化的因素， 鸡蛋这个有吸引力的模型的一个主要方面， 需要仔细研究的是血浆的物理重组 膜组件（例如离子通道）作为轴发生 是成立的。克罗普夫博士最近发现墨角藻属 由年轻的合子制成的原生质体可以用 单向光这提供了直接进入等离子体的途径 膜在轴建立期间。他将审查 细胞表面膜受体的重排 原生质体进行极化和调查离子 墨角藻受精卵膜不对称产生的电流 和在非极性化的原生质体中。最终目标是 监测离子电流、等离子体的空间分布 膜受体和F-肌动蛋白在单细胞中，因为他们建立 一个轴。 %%% 建立一个轴（即哪一端是哪一端） 生物体在发育早期是基本的第一步， 这种有机体的模式形成。这一事件很难 分析植物。Kropf博士正在研究这个过程， 因为它在实验上的优势 相对于许多其他植物。