The Role of a Host ion Pump in RNA Virus Recombination

宿主离子泵在 RNA 病毒重组中的作用

• 批准号: 0817790
• 负责人: Peter Nagy
• 金额: $ 60万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0817790&HistoricalAwards=false
• 关键词: Role; Host; ion; Pump; RNA

Host - virus interactions are greatly affected by the exceptionally rapid rate of virus evolution. Evolution of viruses is frequently driven by genetic recombination, a process that allows viruses to overcome immunity, to infect new host species and change disease symptoms in all types of living organisms. Virus recombination is also a major factor in emergence of new viruses, which is a continuing threat to our society. Therefore, studies on the effect of the host on the viral recombination process are critical to unravel virus evolution, which is currently poorly understood. This project focuses on a recently discovered key host factor affecting RNA recombination in Tomato bushy stunt virus. The host factor is Pmr1p Ca2+/Mn2+ ion-pump, which is a ubiquitous and conserved protein playing critical roles in human genetic diseases, such as Hailey-Hailey Disease and Darier Disease. In order to characterize the role of Pmr1p in viral RNA recombination, the investigator will use advanced genetics tools available for plant and yeast, which is a model host, in combination with biochemical approaches. This research is expected to lead to important new discoveries in viral recombination and contribute key knowledge to the role of the host in virus evolution and virus-host interactions.Broader impact: The research holds promise of benefiting society by leading to groundbreaking results in the area of virus recombination/evolution, host-virus interaction and the emergence of new viruses. The major limitation of current antiviral methods is that they could quickly become ineffective due to rapid virus evolution. Better understanding of viral RNA recombination will have tremendous impact on our abilities to develop better, longer lasting antiviral strategies. Education component: High school and undergraduate students will actively participate in research in the investigator's laboratory.

宿主-病毒相互作用受病毒进化速度的影响很大.病毒的进化通常由基因重组驱动，这一过程使病毒能够克服免疫力，感染新的宿主物种并改变所有类型生物体的疾病症状。病毒重组也是新病毒出现的一个主要因素，这是对我们社会的持续威胁。因此，研究宿主对病毒重组过程的影响对于揭示病毒进化至关重要，而目前对病毒进化的理解还很有限。本项目主要研究番茄丛矮病毒中影响RNA重组的一个关键宿主因子。宿主因子Pmr 1 p Ca 2 +/Mn 2+离子泵是一种普遍存在的保守蛋白，在Hailey-Hailey病和Darier病等人类遗传性疾病中起着重要作用。为了表征Pmr 1 p在病毒RNA重组中的作用，研究人员将使用可用于植物和酵母的先进遗传学工具，酵母是一种模型宿主，结合生物化学方法。该研究有望在病毒重组方面取得重要的新发现，并为宿主在病毒进化和病毒-宿主相互作用中的作用提供关键知识。更广泛的影响：该研究有望在病毒重组/进化、宿主-病毒相互作用和新病毒的出现等领域取得突破性成果，造福社会。目前抗病毒方法的主要局限性是，由于病毒的快速进化，它们可能很快变得无效。更好地了解病毒RNA重组将对我们开发更好，更持久的抗病毒策略的能力产生巨大影响。教育部分：高中生和本科生将积极参与研究者实验室的研究。