CRIF:ID- Development of Instrumentation for the Study of the Spectroscopy and Chemistry of Isolated Biological Ions

CRIF:ID-开发用于分离生物离子的光谱学和化学研究的仪器

• 批准号: 0820766
• 负责人: Timothy Zwier
• 金额: $ 55.97万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0820766&HistoricalAwards=false
• 关键词: CRIF; ID; Development; Instrumentation; Study

This instrument development award from the CRIF-ID program to Timothy S. Zweir and Scott A. McLuckey in the Department of Chemistry at Purdue University will enable them to construct an instrument for the study of ions in the gas phase. Specifically, the instrument will enable tandem mass spectrometry experiments involving ion/ion reactions, ion/molecule reactions, and collisional activation at room temperature. This will be followed by conformation-specific IR and UV spectroscopies in a temperature-controlled ion trap. This instrument promises to allow for new insights into the structures, dynamics, and chemical reactivities of gaseous bio-ions with unique capabilities for examining the role of ion preparation conditions and methodologies. By incorporating or using existing aromatic amino acid residues in the polypeptide, single-conformation IR and UV spectra can be obtained, thereby interrogating the H-bonding architectures of individual conformational isomers and probing their UV spectroscopy and conformation specific fragmentation patterns. The dynamics of conformational isomerization between specific reactant-product conformer pairs can also be probed by selective infrared excitation followed by re-cooling with helium prior to interrogation with a probe UV laser. This collaborative instrument development project combines in a unique way the powerful tools of biological tandem mass spectrometry and optical spectroscopy. The new information generated will likely prove relevant to future progress in molecular biology research via new structural and dynamical insights on gaseous bio-ions. Graduate students will be involved in the project developing skill in the construction and design of state of the art instrumentation.

这项由CRIF-ID计划授予普渡大学化学系的Timothy S.Zweir和Scott A.McLuckey的仪器开发奖将使他们能够建造一种用于研究气相离子的仪器。具体地说，该仪器将实现串联质谱学实验，包括室温下的离子/离子反应、离子/分子反应和碰撞激活。随后将在温度控制的离子陷阱中进行构象特定的IR和UV光谱。该仪器有望对气态生物离子的结构、动力学和化学反应有新的见解，具有独特的能力来检查离子制备条件和方法的作用。通过在多肽中加入或使用现有的芳香族氨基酸残基，可以获得单一构象的IR和UV光谱，从而询问单个构象异构体的氢键结构，并探索它们的UV光谱和构象特定的碎裂模式。特定的反应物-产物构象对之间的构象异构化动力学也可以通过选择性红外激发，然后在用探头紫外激光询问之前用氦重新冷却来探测。这个合作的仪器开发项目以一种独特的方式结合了生物串联质谱学和光学光谱学的强大工具。通过对气态生物离子的新的结构和动力学的洞察，产生的新信息很可能被证明与未来分子生物学研究的进展有关。研究生将参与到该项目中，掌握最先进的仪器设备的建造和设计技能。