Laser Probes of the Spectroscopy and Dynamics of Flexible Biomolecules and Complexes

柔性生物分子和复合物的光谱学和动力学激光探针

• 批准号: 0551075
• 负责人: Timothy Zwier
• 金额: --
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0551075&HistoricalAwards=false
• 关键词: Laser; Probes; Spectroscopy; Dynamics; Flexible

The Experimental Physical Chemistry program supports Timothy S. Zwier of Purdue University and his students in their studies of the single-conformation spectroscopy and conformational dynamics of small biomolecules, peptide mimics, and other small, flexible molecules and complexes cooled in supersonic expansions. These molecules have significant conformational complexity, bridging the gap between systems amenable to gas phase spectroscopy and large biomolecules. The experiments exploit direct infrared absorption or indirect stimulated emission pumping (SEP) to promote selected conformers or isomers to higher energy states. These species then are re-cooled to achieve the sharp spectra necessary for their unambiguous identification and characterization. The research expands the SEP-population transfer techniques into the mid-infrared, allowing the study of a wider range of flexible systems. This work is having a broader impact on problems of protein folding and may provide information about the laser control of molecular shape, with possible applications for using light to store information in single molecules. Graduate and undergraduate students at Purdue University, as well as faculty and students from primarily undergraduate institutions, are participating in the research.

普渡大学的实验物理化学项目支持普渡大学的Timothy S.Zwier和他的学生研究生物小分子、多肽模拟物和其他在超音速膨胀中冷却的灵活小分子和络合物的单构象光谱和构象动力学。这些分子具有显著的构象复杂性，弥合了适用于气相光谱学的体系和大生物分子之间的差距。这些实验利用直接红外吸收或间接受激发射泵浦(SEP)来促进选定的构象或异构体进入更高的能态。然后，这些物种被重新冷却，以获得明确识别和表征所需的锐利光谱。这项研究将SEP-布居转移技术扩展到中红外，允许研究更广泛的灵活系统。这项工作正在对蛋白质折叠问题产生更广泛的影响，并可能提供关于激光控制分子形状的信息，以及利用光在单分子中存储信息的可能应用。普渡大学的研究生和本科生，以及主要是本科院校的教职员工和学生都参与了这项研究。