MRI: Acquisition of a IM-Q-TOF Mass Spectrometer

MRI：购买 IM-Q-TOF 质谱仪

• 批准号: 0821247
• 负责人: Laurence Angel
• 金额: $ 34.2万
• 依托单位: Texas A&M University-Commerce
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821247&HistoricalAwards=false
• 关键词: MRI; Acquisition; IM; TOF; Mass

CBET-0821247AngelThe MRI project involves the acquisition of a state-of-the-art IM-Q-TOF spectrometer will enable a collaboration of faculty from four departments at Texas A&M University-Commerce (A&M Commerce) to conduct cutting-edge biochemical research. The research programs and the training of students from the chemistry, biology and environmental sciences, agriculture, and health and human performance departments will benefit tremendously from this instrument.The project involves seven multidisciplinary collaborations to utilize the unique capabilities of an IM-Q-TOF instrument. Dr. Angel and the Science and Technologies instrument technician, Richard Miller, will manage the IM-Q-TOF and provide necessary training to other collaborators on the operation and capabilities of the instrument. Acquisition of the IM-Q-TOF mass spectrometer will allow students to achieve highly sought-after mass spectrometry training and research experience. These are essential tools in proteomics; a research field that has promising potential for expanding and advancing our knowledge of protein functions and the factors that influence the onset of disease. The research projects made possible through acquisition of an IM-Q-TOF will help faculty integrate research with education and will increase the scientific awareness among the students to adequately prepare them for future scientific/technological opportunities.Intellectual Merit: The acquisition of a state-of-the-art IM-Q-TOF mass spectrometer will advance the knowledge of both faculty and students as carefully designed research projects are developed, conducted and evaluated. The IM-Q-TOF will provide a unique capability for enabling a wide range of research by offering exceptional qualities for bioanalytical chemistry, proteomics and structural studies of mid-tolarge size biomolecules for the research projects involved. The PI will research specific areas using the IM-Q-TOF including: investigating the self-assembly of metal-peptide complexes; characterizing the structures of metal-peptide complexes; measuring the gas-phase, metal-binding affinities of peptides; exploring the energy-resolved, gas-phase reactivity of metal-peptide complexes; determining the solution phase, metal-binding affinities and hydrolysis kinetics of peptides; developing Q-TOF methods for wine quality control. The PI will also support collaborative research detailed in this proposal: Studies of Zn, Cu and Fe Metal-Peptide Complexes; Mass Spectrometric Study of Palladium and Platinum Aqua Complexes as New Proteomics Reagents; Identification of Glycolipids in Stem Cells; Determining Metabolites Induced Under Extreme Exercise; Design and Synthesis of Porphyrin Receptors for Anions and Molecules; Flavonoid Interactions with Segments of Amyloid Beta Peptide and Tau Protein; and, Isolating Scent Chemicals Utilized by Snakes for Hunting Prey.Broader Impact: Advanced mass spectrometry techniques are a vital part of the explosion in knowledge in biochemical research. There are tremendous possibilities for a non-Ph.D. granting institution to attract, retain, and graduate students with sophisticated instrumentation knowledge as well the experience of being a part of significant scientific research. Students and faculty from eighteen 2-year colleges in Northeast Texas will have the opportunity of collaborating with university faculty and students on research projects using this instrument. The total enrollment of these colleges is over 70,000 with 20,000 of these involved in science courses, and over 23% of the students from underrepresented groups. The nature of the instrumental training and research provided by A&M-Commerce will also be fully conducive in providing appropriately trained personnel for the rapidly developing bioanalytical, proteomic, bioengineering and biochemical career markets. The huge requirement for qualified candidates and knowledge in these rapidly growing science fields will be partially supplemented by the research conducted by highly qualified faculty at A&M-Commerce.

CBET-0821247 AngelMRI项目涉及购买一台最先进的IM-Q-TOF光谱仪，这将使德克萨斯农工大学商务部(A&amp；M Commerce)四个系的教职员工能够合作进行尖端的生物化学研究。来自化学、生物和环境科学、农业、卫生和人类行为学系的学生的研究项目和培训将从该仪器中受益匪浅。该项目涉及七个多学科合作，以利用IM-Q-TOF仪器的独特能力。安吉尔博士和科技仪器技术员理查德·米勒将管理IM-Q-TOF，并向其他合作者提供有关仪器操作和能力的必要培训。收购IM-Q-TOF质谱仪将使学生获得备受欢迎的质谱学培训和研究经验。这些都是蛋白质组学中的基本工具；这一研究领域有希望扩大和提高我们对蛋白质功能和影响疾病发生的因素的知识的了解。通过收购IM-Q-TOF实现的研究项目将帮助教师将研究与教育相结合，并将提高学生的科学意识，使他们为未来的科学/技术机会做好充分准备。智力优势：在精心设计的研究项目的开发、实施和评估过程中，收购最先进的IM-Q-TOF质谱仪将提高教师和学生的知识。IM-Q-TOF将为相关研究项目提供生物分析化学、蛋白质组学和中等大小生物分子结构研究的卓越质量，从而为广泛的研究提供独特的能力。PI将使用IM-Q-TOF研究特定领域，包括：研究金属-多肽复合体的自组装；表征金属-多肽复合体的结构；测量多肽的气相、金属结合亲和力；探索金属-多肽复合体的能量分辨、气相反应性；确定多肽的溶液相、金属结合亲和力和水解动力学；开发用于葡萄酒质量控制的Q-TOF方法。PI还将支持本提案中详细介绍的合作研究：锌、铜和铁金属-多肽配合物的研究；作为新的蛋白质组学试剂的钯和铂水配合物的质谱学研究；鉴定干细胞中的糖脂；确定极端运动诱导的代谢物；设计和合成阴离子和分子的卟啉受体；类黄酮类化合物与淀粉样β多肽和Tau蛋白片段的相互作用；以及分离蛇用来捕食猎物的芳香化学品。非授予博士学位的机构有巨大的可能性来吸引、留住和拥有复杂的仪器知识以及作为重大科学研究的一部分的经验的研究生。来自德克萨斯州东北部18所两年制学院的学生和教职员工将有机会使用该仪器与大学教职员工和学生在研究项目上进行合作。这些学院的总招生人数超过7万人，其中2万人涉及理科课程，23%以上的学生来自代表性不足的群体。A&M-Commerce提供的仪器培训和研究的性质也将完全有助于为迅速发展的生物分析、蛋白质组学、生物工程和生化职业市场提供适当培训的人员。在这些快速增长的科学领域，对合格候选人和知识的巨大需求将得到A&M-Commerce高素质教师进行的研究的部分补充。