RUI: Developing Ion Mobility Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition & Redox Activity of Metal Ion Binding Oligopepti
RUI:开发离子淌度质谱技术以确定金属离子识别的结构和机制
基本信息
- 批准号:1764436
- 负责人:
- 金额:$ 21.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-08-01 至 2022-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In this project, funded by the Chemical Structure, Dynamic & Mechanism B Program of the Chemistry Division, Professor Laurence Angel of the Department of Chemistry at Texas A&M University - Commerce is developing new classes of copper and zinc chelating peptides. Disruption of copper and zinc homeostasis is directly implicated in Menkes and Wilson's disease as well as cancers and neurological diseases, such as Alzheimer's and prion disease. Metal chelators are used in the treatment of these types of diseases. The project is well suited for the interdisciplinary training of students in support of the growing bioanalytical, proteomic, bioengineering, and biomedical workforce. Under-represented groups of undergraduate students, including first-generation college students, African-American, Hispanic, and female students, will be encouraged to join the research efforts. More technically, the solution-phase metal ion reactivity of alternative metal-binding peptides (AMBS) and methanobactin (mb-OB3b) is being examined by electrospray ionization ion mobility-mass spectrometry (IM-MS) and density functional theory (DFT). Ultraviolet-visible and fluorescence spectral techniques are being used to check for correlations across gas- and solution-phase analyses to answer the following questions: (1) Which solution-phase behaviors can be examined in the gas phase? (2) Does pH-dependent metal-ion binding result in positively or negatively charged complexes that reflect the solution-phase states? (3) Which sequence residues affect AMBS and mb-OB3b metal-ion binding? (4) Which dissociation mechanisms are involved in the collision-induced dissociation (CID) of AMBS and AMBS cationized by Zn(II) or Cu(I)? (5) Can the metal-ion coordination sites be identified using energy-resolved CID and IM-MS? (6) Can metal-ion displacement be monitored by IM-MS titrations and collision cross-section changes? (7) Can IM-MS analyses and theoretical collision cross-sections identify which type of coordination each metal ion prefers? (8) How do IM-MS, DFT, UV-Vis, and fluorescence findings correlate? The study could discover new reaction mechanisms and develop more effective IM-MS molecular analysis techniques, while moving toward the design of chelating agents for treating diseases related to metal-ion imbalance.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在这个项目中,由化学部的化学结构,动力学机制B计划资助,德克萨斯A M大学化学系的Laurence Angel教授正在开发新的铜和锌螯合肽类。铜和锌稳态的破坏直接涉及门克斯和威尔逊病以及癌症和神经系统疾病,如阿尔茨海默病和朊病毒病。金属螯合剂用于治疗这些类型的疾病。 该项目非常适合学生的跨学科培训,以支持不断增长的生物分析,蛋白质组学,生物工程和生物医学劳动力。 代表性不足的本科生群体,包括第一代大学生,非洲裔美国人,西班牙裔和女学生,将被鼓励加入研究工作。 更技术性地,替代金属结合肽(AMBS)和甲烷氧化菌素(mb-OB 3b)的溶液相金属离子反应性正在通过电喷雾离子迁移率质谱(IM-MS)和密度泛函理论(DFT)进行检查。紫外-可见和荧光光谱技术被用来检查气相和溶液相分析的相关性,以回答以下问题:(1)哪些溶液相行为可以在气相中检查?(2)pH依赖的金属离子结合是否会导致反映溶液相状态的带正电荷或负电荷的复合物?(3)哪些序列残基影响AMBS和mb-OB 3b金属离子结合?(4)AMBS和Zn(II)或Cu(I)阳离子化AMBS的碰撞诱导解离(CID)涉及哪些解离机制?(5)金属离子的配位位点能用能量分辨CID和IM-MS鉴定吗?(6)金属离子置换可以通过IM-MS滴定和碰撞截面变化来监测吗?(7)IM-MS分析和理论碰撞截面能否确定每种金属离子更喜欢哪种配位类型?(8)IM-MS、DFT、UV-Vis和荧光结果如何相互关联? 该研究可以发现新的反应机制,开发更有效的IM-MS分子分析技术,同时朝着设计用于治疗与金属离子失衡相关疾病的螯合剂的方向发展。该奖项反映了NSF的法定使命,通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Thermochemical and conformational studies of Ni(II) and Zn(II) ternary complexes of alternative metal binding peptides with nitrilotriacetic acid
替代金属结合肽与次氮基三乙酸的 Ni(II) 和 Zn(II) 三元复合物的热化学和构象研究
- DOI:10.1016/j.ijms.2021.116792
- 发表时间:2022
- 期刊:
- 影响因子:1.8
- 作者:Flores, Amber A.;Arredondo, Anna V.;Corrales, Anna J.;Duvak, Chloe L.;Mitchell, Charles L.;Falokun, Oladapo;Aguilar, Cynthia L.;Kim, Aram;Daniel, Bency C.;Karabulut, H. Derya
- 通讯作者:Karabulut, H. Derya
Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
确定金属结合寡肽的金属离子识别和氧化还原活性的结构和机制的离子淌度-质谱技术
- DOI:10.3791/60102
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Yousef, Enas N.;Sesham, Ramakrishna;McCabe, Jacob W.;Vangala, Rajpal;Angel, Laurence A.
- 通讯作者:Angel, Laurence A.
ESI-IM-MS reveals the metal binding of three analog methanobactin peptides with different numbers of free Cys at physiological pH
ESI-IM-MS 揭示了三种类似甲烷菌素肽在生理 pH 下与不同数量的游离半胱氨酸的金属结合
- DOI:10.1016/j.ijms.2021.116640
- 发表时间:2021
- 期刊:
- 影响因子:1.8
- 作者:Vangala, Rajpal;Angel, Laurence A.
- 通讯作者:Angel, Laurence A.
Thermochemical Studies of Ni(II) and Zn(II) Ternary Complexes using Ion Mobility-Mass Spectrometry
使用离子淌度-质谱法对 Ni(II) 和 Zn(II) 三元配合物进行热化学研究
- DOI:10.3791/63722
- 发表时间:2022
- 期刊:
- 影响因子:0
- 作者:Corrales, Anna J.;Arredondo, Anna V.;Flores, Amber A.;Duvak, Chloe L.;Mitchell, Charles L.;Spezia, Riccardo;Angel, Laurence A.
- 通讯作者:Angel, Laurence A.
Weak Acid-Base Interactions of Histidine and Cysteine Affect the Charge States, Tertiary Structure, and Zn(II)-Binding of Heptapeptides
组氨酸和半胱氨酸的弱酸碱相互作用影响七肽的电荷状态、三级结构和 Zn(II) 结合
- DOI:10.1007/s13361-019-02275-7
- 发表时间:2019
- 期刊:
- 影响因子:3.2
- 作者:Lin, Yu-Fu;Yousef, Enas N.;Torres, Efren;Truong, Linh;Zahnow, James M.;Donald, Cole B.;Qin, Ying;Angel, Laurence A.
- 通讯作者:Angel, Laurence A.
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Laurence Angel其他文献
Laurence Angel的其他文献
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{{ truncateString('Laurence Angel', 18)}}的其他基金
RUI: Thermochemistry, Reaction Dynamics and Conformational Changes that Accompany the Collisional Activation of Peptide Ternary Complexes and Recombinant Tagged Proteins
RUI:肽三元复合物和重组标记蛋白碰撞激活时的热化学、反应动力学和构象变化
- 批准号:
2247511 - 财政年份:2023
- 资助金额:
$ 21.63万 - 项目类别:
Standard Grant
MRI: Acquisition of a IM-Q-TOF Mass Spectrometer
MRI:购买 IM-Q-TOF 质谱仪
- 批准号:
0821247 - 财政年份:2008
- 资助金额:
$ 21.63万 - 项目类别:
Standard Grant
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