Investigating the Stabilization of Biological Systems with Clathrate Hydrates

研究笼形水合物生物系统的稳定性

• 批准号: 0828649
• 负责人: Amadeu Sum
• 金额: $ 4.97万
• 依托单位: Colorado School of Mines
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0828649&HistoricalAwards=false
• 关键词: Investigating; Stabilization; Biological; Systems; Clathrate

CBET-0828649A. Sum, Colorado School of MinesThis exploratory one-year proposal investigates a new frontier for the fundamental understanding and application of clathrate hydrates in the stabilization of biological systems. Stabilization of biological systems, or biopreservation, is of major importance for the storage and preservation of cells and proteins in biology, biotechnology, medicine, and food technology. Clathrate hydrates are inclusion compounds formed from the hydrogen bonding of water molecules that enclose small, mostly non-polar compounds. The benefits of employing clathrate hydrates over current stabilization approaches, namely lyophilization (removal of water) and cryopreservation (cold storage), are the preservation of biological systems at fully hydrated conditions and the ability for stabilization at temperatures above the normal freezing point of water.The intellectual merit of this novel study is to identify suitable clathrate hydrate formers to stabilize biological materials and to perform viability studies on biological systems to demonstrate the suitability of clathrate hydrate in their stabilization. The experimental measurements will be performed in a custom-designed cell suitable for hydrate formation and biological studies. Measurement of hydrate formation conditions will concentrate on hydrate formers that are benign to biological systems (cells and proteins) and have equilibrium formation above the normal freezing point of water (0?aC). Some of the hydrate formers to be considered in this study include mixtures of xenon and carbon dioxide with tetrahydrofuran and ethylene oxide. Measurements will be performed in both neat water and biological media. These measurements will serve as the basis to study the stabilization of biological systems with clathrate hydrates, which will be initially verified by the activity and viability of microorganisms and animal cells.This exploratory study is transformative, as it introduces novel concepts in the area of clathrate hydrates and new approaches for the stabilization of biological systems. The broader impact component of the project is the investigation of a unexplored area of research with potential applications in the stabilization of cellular and protein systems, in the development of new food processing and preservation technologies, in the encapsulation of small organic agents, and in the mechanism for anesthesia and hibernation. The project will demonstrate the unique properties of clathrate hydrates in biology, which is an area beyond its well-established areas of research and applications (i.e., energy, storage, and environment). This project will also include training of a graduate student, who will work closely with the PI and his collaborator, Prof. Y. Martin Lo at the University of Maryland-College Park.

CBET-0828649A。科罗拉多矿业学院这一探索性的为期一年的建议调查了一个新的前沿的基本理解和应用的笼形水合物在生物系统的稳定。生物系统的稳定或生物保存对于生物学、生物技术、医学和食品技术中细胞和蛋白质的储存和保存具有重要意义。 笼形水合物是由水分子的氢键形成的包合化合物，所述水分子包封小的、大部分非极性化合物。 采用笼形水合物优于目前的稳定方法，即冻干的益处（除水）和冷冻保存（冷藏），是生物系统在完全水合条件下的保存和在高于水的正常凝固点的温度下稳定的能力。这项新研究的智力价值是确定合适的笼形水合物形成剂，以稳定生物材料，对生物系统的可行性研究，以证明笼形水合物在其稳定中的适用性。 实验测量将在定制设计的适合水合物形成和生物研究的单元中进行。水合物形成条件的测量将集中在对生物系统（细胞和蛋白质）无害的水合物形成剂上，并且在水的正常凝固点（0？aC）。本研究中考虑的一些水合物形成剂包括氙和二氧化碳与四氢呋喃和环氧乙烷的混合物。将在纯净水和生物介质中进行测量。这些测量结果将作为研究笼形水合物生物系统稳定性的基础，初步将通过微生物和动物细胞的活性和生存能力进行验证。这项探索性研究具有变革性，因为它引入了笼形水合物领域的新概念和生物系统稳定性的新方法。该项目的更广泛影响部分是调查一个未探索的研究领域，其潜在应用包括细胞和蛋白质系统的稳定、新食品加工和保存技术的开发、小有机制剂的封装以及麻醉和冬眠机制。该项目将展示笼形水合物在生物学中的独特性质，这是一个超越其成熟的研究和应用领域的领域（即，能源、存储和环境）。这个项目还将包括一名研究生的培训，他将与PI及其合作者Y教授密切合作。马里兰大学帕克分校的马丁·罗。