Role of perifibrillar proteins in collagen fibril assembly, fibril interactions and matrix remodelling

原纤维周围蛋白在胶原原纤维组装、原纤维相互作用和基质重塑中的作用

• 批准号: 190038206
• 负责人: Professor Dr. Frank Zaucke
• 金额: --
• 依托单位: Klinik für Orthopädie (Friedrichsheim)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/190038206?language=en
• 关键词: Role; perifibrillar; proteins; collagen; fibril

The cartilage extracellular matrix contains two major suprastructures, namely the fibrillar collagenous network and gel-like aggrecan complexes. In order to guarantee tissue stability and function a mutual interaction between these two supramolecular compartments is required. This is achieved by a set of molecules present on the collagen fibril surface, the so-called perifibrillar proteins. These proteins and proteoglycans are often organized in higher order complexes. Their biological relevance is underlined by the fact that mutations in their encoding genes can lead to chondrodysplasias or predispose for degenerative diseases, such as osteoarthritis. In the current project, we will characterize the role of perifibrillar proteins in fibril assembly, fibril interactions and matrix remodelling. Protein biochemistry, electron microscopy, chondrocyte culture and mouse models deficient in perifibrillar proteins will be employed to analyze individual components and their contribution to the overall structure and function of adaptor complexes. This will give insight into the role of perifibrillar proteins in cartilage homeostasis during normal development and possibly into mechanisms involved in cartilage diseases.

软骨细胞外基质包含两个主要的上层构造，即纤维胶原网络和类似凝胶的脂质综合体。为了确保组织稳定性和功能，需要这两个超分子室之间的相互作用。这是通过胶原原纤维表面上的一组分子（所谓的peifibrillar蛋白）实现的。这些蛋白质和蛋白聚糖通常是在高阶复合物中组织的。它们的生物学相关性强调了以下事实：其编码基因中的突变会导致退行性疾病（例如骨关节炎）导致软骨发育不全或易感性。在当前的项目中，我们将表征peifibrillar蛋白在原纤维组件，原纤维相互作用和基质重塑中的作用。蛋白质生物化学，电子显微镜，软骨细胞培养和小鼠模型缺乏蛋白质的蛋白质将用于分析单个成分及其对适配器复合物的整体结构和功能的贡献。这将洞悉正常发育过程中蛋白纤维蛋白在软骨稳态中的作用，并可能涉及与软骨疾病有关的机制。

项目成果

Collagen II regulates chondroycte integrin expression profile and differentiation

• DOI: 10.3109/03008207.2015.1026965
• 发表时间: 2015-08-01
• 期刊: CONNECTIVE TISSUE RESEARCH
• 影响因子: 2.9
• 作者: Xin, Wei;Heilig, Juliane;Zaucke, Frank
• 通讯作者: Zaucke, Frank