Arabidopsis 2010: Regulatory mechanisms of energy signaling in plants

项目摘要Jen SheenIOS-0843244标题：拟南芥2010：植物中能量信号的调节机制光合成植物是维持地球上生命的主要太阳能转换器。尽管它具有基本的重要性，但人们对植物如何在日常的明暗循环中感知和适应黑暗，或对影响光合作用和呼吸作用并耗尽能源供应的不可预测的环境压力知之甚少。最近的研究发现，在进化上保守的拟南芥蛋白激酶KIN10和KIN11控制着转录的收敛重编程，以响应似乎不相关的黑暗、糖和胁迫条件。该项目旨在通过揭示保守的能量感受器的不同生理和发育功能、信号链接和调节机制来构建植物能量信号的新概念框架。可诱导突变转基因株系将被用来对能量信号在植物对不同环境挑战的反应和适应中的作用以及在植物生长发育中的作用获得新的见解。综合运用细胞、生化、遗传和基因组的方法，从控制传感器的活性、稳定性、定位、伙伴相互作用到下游转录因子和主要靶基因，来加强我们对能量信号的调控机制的理解。这个项目代表了一条新的研究路线，将不同的植物反应和调控整合到对植物生长和生存至关重要的聚合能量信号中。鉴于全球对发展可再生生物燃料的需求日益增加，对能源信号调节机制的研究具有迫切的重要性。基础研究将能够有针对性地对碳分配、生长发育、结构以及压力和病原菌抗性进行基因改造，这些都是作物产量和可再生能源生产的主要决定因素。该项目将为学生和博士后提供独特的多学科和综合研究培训机会，并促进女性和少数族裔科学家成为未来的研究领导者。该项目的首席研究员提供服务于植物群落和公众的教育和推广计划。拟南芥2010 projecthttp://genetics.mgh.harvard.edu/sheenweb/main_page.html的KIN10 AT3g01090、KIN11 AT3g29160、KINB1 AT5g21170、KINB2 AT4g16360、KINB3 AT2g28060、KINBG AT1g09020、KINBG At3g48530URL所选基因网络的描述

PROJECT ABSTRACTJen SheenIOS-0843244Title: Arabidopsis 2010: Regulatory Mechanisms of Energy Signaling in PlantsPhotosynthetic plants are the principal solar energy converter sustaining life on Earth. Despite its fundamental importance, little is known about how plants sense and adapt to darkness in the daily light-dark cycle or to unpredictable environmental stresses that compromise photosynthesis and respiration and deplete energy supplies. Recent studies have discovered that the evolutionarily conserved Arabidopsis protein kinases, KIN10 and KIN11, control convergent reprogramming of transcription in response to seemingly unrelated darkness, sugar and stress conditions. The project aims to build a new conceptual framework of energy signaling in plants by uncovering the diverse physiological and developmental functions, signaling links, and regulatory mechanisms of the conserved energy sensors. Inducible mutant transgenic lines will be use to gain new insight into the roles of energy signaling in plant response and adaptation to diverse environmental challenges, and in plant growth and development. Integrative cellular, biochemical, genetic and genomic approaches will be applied to enhance our understanding of the regulatory mechanisms of energy signaling from the control of sensor activity, stability, localization, partner interactions to its downstream transcription factors and primary target genes. This project represents a new line of research that integrates diverse plant responses and regulation into convergent energy signaling vital to plant growth and survival. The studies on the regulatory mechanisms of energy signaling are urgently important in light of the rising global needs in the development of renewable biofuels. The basic research will enable targeted genetic modification of carbon allocation, growth and development, architecture, and stress and pathogen resistance, all major determinants of crop yield and renewable energy production. The project will provide unique training opportunities for students and postdocs in multidisciplinary and integrative research, and promote woman and minority scientists as future research leaders. The Principal Investigator of the project offers educational and outreach programs serving the plant community and the general public.Description of the network of genes selectedKIN10 At3g01090, KIN11 At3g29160, KINB1 At5g21170, KINB2 At4g16360, KINB3 At2g28060, KINBG At1g09020, KING At3g48530URL of the Arabidopsis 2010 projecthttp://genetics.mgh.harvard.edu/sheenweb/main_page.html