Molecular Genetic and Cellular Analysis of Light Response inMaize Leaf Cells

小行星9407834 光调节植物生长发育的许多方面，其中黑暗生长的黄化叶片转化为光合能力强的绿色叶片（绿化）是植物生命最重要的，并在高等植物中得到了最好的记载。 虽然红光和蓝光光感受器，光敏色素和隐花色素，已牵连在光信号的感知，很少有人知道的细胞内信号转导途径介导的光诱导的发育过程标记叶绿素积累和光合基因激活的分子基础。 我们感兴趣的是阐明介导光反应的分子机制，在叶细胞的玉米实验系统提供的其他生物化学，细胞和分子方法相结合的遗传功率。 我们将系统地研究光诱导基因表达和叶绿体生物发生缺陷的玉米突变体。 一个通用的和强大的erce细胞瞬时表达系统将被用来克隆和表征调控基因的光诱导基因表达和叶绿体生物合成，这是细胞自主活动。 这些研究将有助于我们了解高等植物的光合功能和信号传导。 光调节植物生长和发育的许多方面，其中黑暗生长的黄化叶转化为光合能力强的绿色叶（绿化）是植物生命最重要的，并且在高等植物中有最好的记载。 虽然红光和蓝光光感受器，光敏色素和隐花色素，已牵连在光信号的感知，很少有人知道的细胞内信号转导途径介导的光诱导的发育过程标记叶绿素积累和光合基因激活的分子基础。 我们有兴趣阐明介导光反应的分子机制，在叶片细胞的玉米实验系统提供的其他生物化学，细胞和分子方法相结合的遗传功率。 我们计划通过RT-PCR和原生质体瞬时表达分析系统地分析现有的玉米光合突变体。 我们将构建表达cDNA文库，并进行初步实验，以探索利用原生质体瞬时表达技术进行突变体互补的可能性。 新的细胞表面报告基因和荧光激活的原生质体分选技术，为表达克隆和突变体互补提供了效率和高分辨率，将进行测试。这可能是一个很好的工具，以解开意想不到的新见解光信号转导和基因调控的分子机制在高等植物。 ***

9407834 Sheen Light regulates many aspects of plant growth and development among which the conversion of dark-grown etiolated leaves to photosynthetic competent green leaves (greening) is most essential to plant life and best documented in higher plants. Although both red and blue light photoreceptors, phytochrome and cryptochrome, have been implicated in light signal perception, very little is known about the molecular basis of the intracellular signal transduction pathways mediating the light-inducible developmental processes marked by chlorophyll accumulation and photosynthetic gene activation. We are interested in elucidating the molecular mechanisms mediating light responses in leaf cells by combining the genetic power with other biochemical, cellular and molecular approaches offered by the maize experimental system. We will investigate systematically maize mutants that are defective in light-inducible gene expression and chloroplast biogenesis. A versatile and powerful singlecell transient expression system will be used to clone and characterize regulatory genes controlling light-inducible gene expression and chloroplast biogenesis which are cell autonomous activities. The studies will facilitate our understanding of photosynthetic functions and signal transduction in higher plants. %%% Light regulates many aspects of plant growth and development among which the conversion of dark-grown etiolated leaves to photosynthetic competent green leaves (greening) is most essential to plant life and best documented in higher plants. Although both red and blue light photoreceptors, phytochrome and cryptochrome, have been implicated in light signal perception, very little is known about the molecular basis of the intracellular signal transduction pathways mediating the light-inducible developmental processes marked by chlorophyll accumulation and photosynthetic gene activation. We are interested in elucidating the molecular mechanisms mediating light respo nses in leaf cells by combining the genetic power with other biochemical, cellular and molecular approaches offered by the maize experimental system. We are planning to analyze systematically the existing maize photosynthetic mutants by RT-PCR and protoplast transient expression assays. We will make expression cDNA library and conduct pilot experiments to explore the possibility of complementing mutants using the protoplast transient expression assay. New cell surface reporter genes and fluorescence activated protoplast sorting techniques which offer the efficiency and high resolution for expression cloning and mutant complementation will be tested. It is likely that the system will serve as an excellent tool to unravel unexpected new insights into the molecular mechanisms of light signal transduction and gene regulation in higher plants. ***