CAREER: Characterization of new Arabidopsis mutants with altered response to auxin

职业：对生长素反应改变的新拟南芥突变体的表征

• 批准号: 0845305
• 负责人: Nihal Dharmasiri
• 金额: --
• 依托单位: Texas State University - San Marcos
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0845305&HistoricalAwards=false
• 关键词: CAREER; Characterization; new; Arabidopsis; mutants

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Some of the major challenges that the world faces today are how to feed the ever-increasing population and to find the ways to deal with global climatic change. A better understanding of molecular mechanisms involved in plant growth and development is necessary to design new strategies to improve crop plants that suit the changing environment. The plant hormone auxin plays a pivotal role in growth and development, regulating many developmental programs. Although molecular mechanisms involved in auxin signaling in plants have been studied in great detail, our understanding on these molecular mechanisms is far from complete. Synthetic chemicals that perturb signaling pathways have been used to identify new genes and their functions in these signaling pathways. This study will focus on identification and characterization of three Arabidopsis mutants, pic7, pic30 and pic115 with altered response to picloram, an auxinic herbicide that is widely used in Agriculture. During the term of this project, these three mutant genes will be identified by map-based cloning, and their functions in auxin response will be characterized using genetic, molecular and biochemical approaches. As picloram functions differently from other known auxins, it is expected that these genes and their functions will elucidate new molecular mechanisms involved in plant auxin response. The broader impacts of this work will enhance science education at different levels. The project is set to train graduate, undergraduate and high school students in auxin biology research during the term of this project. Texas State University has 27% minority student enrollment and nearby San Marcos High School (SMHS) has 64% Hispanic student population. The project will have a major impact on training of minority students in science. Additionally, an inquiry based semester?long laboratory module will be conducted with the undergraduate students in Bio 3465 Plant Physiology course. To maximize the impact of the research on grade 6-12 education, an inquiry module on auxin biology will be introduced to pre and in-service teacher training at the Texas State University. In collaboration with the SMHS, one to two high school students will be trained during each summer. As experiments outlined here involve several disciplines such as genetics, molecular biology, biochemistry, physiology and genomics, students will be exposed to hands on experiences in many scientific disciplines.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。当今世界面临的一些主要挑战是如何养活不断增长的人口，以及如何应对全球气候变化。更好地了解植物生长发育的分子机制对于设计新的策略来改善作物以适应不断变化的环境是必要的。植物激素生长素在植物生长发育中起着关键作用，调节着许多发育程序。虽然植物生长素信号传导的分子机制已经得到了详细的研究，但我们对这些分子机制的了解还很不完整。干扰信号通路的合成化学物质已被用于识别新基因及其在这些信号通路中的功能。本研究将重点鉴定和鉴定三种拟南芥突变体pic7、pic30和pic115，它们对picloram（一种广泛应用于农业的抗氧化除草剂）的反应发生了改变。在本项目期间，将通过图谱克隆的方法对这三个突变基因进行鉴定，并利用遗传学、分子生物学和生物化学方法对其在生长素反应中的功能进行表征。由于picloram的功能不同于其他已知的生长素，因此这些基因及其功能有望阐明植物生长素反应的新分子机制。这项工作的更广泛影响将加强不同层次的科学教育。本项目旨在培养研究生、本科生和高中生在生长素生物学研究方面的能力。德克萨斯州立大学有27%的少数族裔学生，附近的圣马科斯高中有64%的西班牙裔学生。这个项目将对培养少数民族学生的科学能力产生重大影响。此外，一个以探究为基础的学期？长时间的实验模块将与生物3465植物生理学课程的本科生一起进行。为了使研究对6-12年级的教育产生最大的影响，将在德克萨斯州立大学的职前和在职教师培训中引入生长素生物学的探究模块。与SMHS合作，每年夏天将培训一至两名高中生。由于这里概述的实验涉及几个学科，如遗传学、分子生物学、生物化学、生理学和基因组学，学生将接触到许多科学学科的实践经验。