New catalytic functions for [4Fe-4S] clusters

[4Fe-4S]簇的新催化功能

• 批准号: 0848196
• 负责人: Eduardus Duin
• 金额: $ 42.4万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2013-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0848196&HistoricalAwards=false
• 关键词: New; catalytic; functions; 4Fe; 4S

This Research award in the Inorganic, Bioinorganic and Organometallic Chemistry program and the Molecular and Cellular Biology program supports work by Professor Eduardus (Evert) C. Duin at Auburn University, Alabama, to probe the reaction mechanism of the penultimate and ultimate proteins in the 1-deoxy-D-xylulose-5-phosphate (DOXP) pathway, GcpE and LytB, and the role of their active-site iron-sulfur clusters in this process. Kinetic experiments will provide insight in the role of several transient EPR-active species in the reaction mechanisms. The species will be characterized using the combination of EPR/ENDOR spectroscopies and isotopic labeling of selected atoms in the substrate structures. In addition further details on the mechanisms of the enzymes will be obtained through substrate modulation studies. The combined data will provide a complete understanding of the mechanisms of the GcpE and LytB enzymes. Since both enzymes seem to be involved in a completely new type of catalysis this will be an important contribution to the iron-sulfur field. In addition, this knowledge will benefit the commericialization of biotechnology through increased ease of production of commercially interesting isoprenoids and the development of novel herbicides that are less harmful to humans. The funding also supports the Biochemistry division research and teaching efforts through the acquisition of equipment for rapid-mix/rapid freeze studies.

这个研究奖在无机，生物无机和有机化学程序和分子和细胞生物学程序支持教授Eduardus（Evert）C。Duin在亚拉巴马的奥本大学的研究，以探索1-脱氧-D-木酮糖-5-磷酸（DOXP）途径中倒数第二和最后两种蛋白质GcpE和LytB的反应机制，以及它们的活性位点铁硫簇在这一过程中的作用。动力学实验将提供几个瞬态EPR活性物种在反应机制中的作用的见解。将使用EPR/ENDOR光谱和底物结构中选定原子的同位素标记的组合来表征物质。此外，将通过底物调节研究获得酶机制的进一步细节。综合数据将提供对GcpE和LytB酶机制的完整理解。由于这两种酶似乎都参与了一种全新的催化类型，这将是对铁硫领域的重要贡献。此外，这方面的知识将有利于生物技术的商业化，通过增加生产商业上感兴趣的类异戊二烯的容易性和开发对人类危害较小的新型除草剂。这笔资金还通过购买快速混合/快速冷冻研究设备来支持生物化学部门的研究和教学工作。