Biophysics of the bacterial nucleoid: Structure, replication, and segregation of the E. coli chromosome

细菌核的生物物理学：大肠杆菌染色体的结构、复制和分离

• 批准号: 0848451
• 负责人: Paul Wiggins
• 金额: $ 60万
• 依托单位: Whitehead Institute for Biomedical Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0848451&HistoricalAwards=false
• 关键词: Biophysics; bacterial; nucleoid; Structure; replication

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).In this project the PI proposes to measure, analyze, and model chromosome conformation based on the imaging of an extensive collection of fluorescently-labeled strains in prokaryotic cells. The PI will also introduce the co-variance matrix for loci displacement technique and use it to characterize and quantitatively measure the structural and mechanical properties of the nucleoid and its cellular tethering. The mechanism of nucleoid organization and segregation by quantitatively characterizing and modeling the effect of partitioning mutants and drugs on nucleoid structure using locus-distribution-function, covariance-matrix, and time-lapse-based techniques will be studied as well. Graduate students, visiting students, and postdocs working with the PI at the Whitehead Institute for Biomedical Research and the co-PI at the Department of Physics at Brandeis University on the problems outlined in this proposal will work in an interdisciplinary environment and will learn how to combine cell-biology and genetic, and bioinformatic techniques with single-molecule, theoretical and computational biophysics. The proposed research will be tightly coupled to the further development of courses for the undergraduate Biological Physics major and the graduate Quantitative Biology program at Brandeis, initiated by the co-PI, as well as working on the second edition of the textbook Physical Biology of the Cell.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。在这个项目中，PI建议测量，分析，并基于原核细胞中大量荧光标记菌株的成像来模拟染色体构象。PI还将引入基因座位移技术的协方差矩阵，并使用它来表征和定量测量类核及其细胞系索的结构和力学性能。通过使用位点分布函数、协方差矩阵和基于延时的技术定量表征和模拟分配突变体和药物对类核结构的影响，研究类核组织和分离的机制。怀特黑德生物医学研究所的PI和布兰迪斯大学物理系的联合PI的研究生、访问学生和博士后将在跨学科的环境中工作，并将学习如何将细胞生物学和遗传学、生物信息学技术与单分子、理论和计算生物物理学结合起来。拟议的研究将与布兰代斯大学生物物理专业本科课程和研究生定量生物学项目的进一步发展紧密结合，该项目由联合pi发起，并致力于第二版教科书《细胞物理生物学》的编写。