IDBR: Controlling Oxygen in Standard Multiwell Plates with a Microfabricated Add-on

IDBR:使用微加工附加装置控制标准多孔板中的氧气

基本信息

  • 批准号:
    0852416
  • 负责人:
  • 金额:
    $ 50万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-05-01 至 2013-04-30
  • 项目状态:
    已结题

项目摘要

Oxygen is a key metabolic variable that influences many different biological phenomena. However, current tools to probe this variable are inefficient and have not changed since the dawn of cell culture techniques. Yet, as oxygen is increasingly implicated in many signaling pathways, enabling a simple tool to explore this would greatly facilitate these investigations and accelerate scientific discovery. In this project, a platform to independently control local gas concentrations within each well of a multiwell plate will be developed and optimized. The platform will consist of a polydimethylsiloxane (PDMS) insert that will nest into a standard multiwell plate. The insert is a passive network with a gas permeable membrane aimed to deliver gas to adherent cell cultures in multiwell plates. The insert will act as a sink or source of oxygen depending on the concentration of the oxygen in the microchannels and will be immersed in the cell media of the well. The gas will be delivered to the cell monolayer through simple diffusion of oxygen across a PDMS membrane and dissolve into the culture media of the multiwell plate. This close spacing between the PDMS membrane and cell monolayer allows for rapid diffusion to impose steady state gradients of oxygen across the well within minutes. The device will not only enable control of oxygen gradients, but will lead to a whole class of devices that will allow researchers to precisely control the microenvironment in multiwell plates to address a variety of The platform has potential to facilitate new and exciting experiments not possible with current techniques, by replicating the oxygen gradients that occur in living systems (in vivo) in a laboratory model (in vitro) accessible to any standard cell biology lab. The result would have a huge impact across many fields.To develop interest in science and engineering, underrepresented minority elementary students will be exposed to interactive laboratory activities at the University of Illinois at Chicago. At risk elementary school students will be targeted by working with the Girl Scouts of Chicago to provide positive experiences with science and engineering. Program evaluation will include both formative feedback, which will allow us to adjust our methods as we learn from the activities, and a summative appraisal of whether the stated goals have been met.
氧气是一个关键的代谢变量,影响许多不同的生物现象。然而,目前的工具来探测这个变量是低效的,并没有改变,因为黎明的细胞培养技术。然而,随着氧气越来越多地参与许多信号通路,使一个简单的工具来探索这将大大促进这些调查和加速科学发现。在该项目中,将开发和优化一个平台,以独立控制多孔板每个孔内的局部气体浓度。该平台将由聚二甲基硅氧烷(PDMS)插入物组成,该插入物将嵌入标准多孔板中。插入物是具有透气膜的被动网络,旨在将气体递送至多孔板中的贴壁细胞培养物。根据微通道中氧的浓度,插入物将充当氧的槽或源,并将浸入孔的细胞培养基中。气体将通过氧气穿过PDMS膜的简单扩散递送至细胞单层,并溶解到多孔板的培养基中。PDMS膜和细胞单层之间的这种紧密间隔允许快速扩散,以在几分钟内在孔中施加稳态氧梯度。该设备不仅能够控制氧气梯度,而且将导致一类设备,使研究人员能够精确地控制多孔板中的微环境,以解决各种各样的平台有潜力促进新的和令人兴奋的实验,而这些实验是当前技术所不可能实现的,通过在任何标准细胞生物学实验室可访问的实验室模型(体外)中复制在生命系统(体内)中发生的氧梯度。其结果将对许多领域产生巨大影响。为了培养对科学和工程的兴趣,代表性不足的少数民族小学生将接触到芝加哥伊利诺伊大学的互动实验室活动。 处于危险之中的小学生将与芝加哥的女童子军合作,提供科学和工程方面的积极经验。 计划评估将包括形成性反馈,这将使我们能够调整我们的方法,因为我们从活动中学习,并总结评估是否达到了既定的目标。

项目成果

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David Eddington其他文献

El abundante agua fría: Hermaphroditic Spanish Nouns
El丰富的agua fría:雌雄同体的西班牙语名词
Surface analogy and spelling rules in English vowel alternations
英语元音交替中的表面类比和拼写规则
  • DOI:
  • 发表时间:
    2001
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Eddington
  • 通讯作者:
    David Eddington
Spanish diminutive formation without rules or constraints
没有规则或限制的西班牙语小写形式
  • DOI:
    10.1515/ling.2002.017
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    1.1
  • 作者:
    David Eddington
  • 通讯作者:
    David Eddington
Analogical Modeling : An Update
类比建模:更新
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Eddington;Deryle W. Lonsdale
  • 通讯作者:
    Deryle W. Lonsdale
Issues in modeling language processing analogically
类比建模语言处理的问题
  • DOI:
    10.1016/s0024-3841(03)00063-9
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    1.1
  • 作者:
    David Eddington
  • 通讯作者:
    David Eddington

David Eddington的其他文献

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{{ truncateString('David Eddington', 18)}}的其他基金

EAGER Collaborative Proposal: A microfluidic platform for the discovery of new, life-like chemical systems
EAGER 合作提案:用于发现新的、逼真的化学系统的微流体平台
  • 批准号:
    1624413
  • 财政年份:
    2016
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant
IDBR-B: Precise Oxygen Landscapes for Cells and Tissues in Culture
IDBR-B:培养细胞和组织的精确氧气景观
  • 批准号:
    1253060
  • 财政年份:
    2013
  • 资助金额:
    $ 50万
  • 项目类别:
    Continuing Grant
Collaborative Research: Microfluidics for Multiple Engineering Disciplines
协作研究:多工程学科的微流控
  • 批准号:
    0814375
  • 财政年份:
    2008
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant
SGER: Exemplar-based Modeling of Spanish Language Phenomena
SGER:基于范例的西班牙语语言现象建模
  • 批准号:
    0082195
  • 财政年份:
    2000
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant

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